链脲佐菌素诱导1型糖尿病大鼠模型的影响因素探讨

Factors Affacting the 1 tape Diabetic Model Induced by Streptozocin in Rats

通过注射链脲佐菌素(STZ)制作大鼠1型糖尿病模型,观察大鼠品系、给药剂量、给药次数对大鼠成模率、死亡率的影响,同时研究利用口服葡萄糖耐量试验(OGTT)判断大鼠糖尿病成模率的意义。随机取16只SD大鼠设置正常对照组。试验一:Wistar大鼠随机分为中剂量组(55 mg/kg,n=12)和高剂量组(65 mg/kg,n=12);试验二:SD大鼠一次性腹腔注射STZ(55 mg/kg,n=12),与1中的Wistar大鼠作对比;试验三:SD大鼠随机分为一次给药组(n=16)和两次给药组(n=16),注射剂量均为55 mg/kg,观察期间进行OGTT。结果表明,Wistar大鼠成模率和死亡率均高于SD大鼠;采用SD大鼠、中剂量给药和两次给药的方式可提高成模率,并降低死亡率;在有明确胰岛病理改变的模型组大鼠,其OGTT异常阳性率显著高于空腹血糖异常阳性率。用STZ诱导糖尿病模型是一种稳定可靠的方法。

To investigate the effects of streptozocin （STZ） dosage, STL administration way ana me variety of rats on the incidence and mortality of tape 1 diabetes, and to study the value of oral glucose tolerance test （OGTT） for making an estimate of the incidence of diabetes mellitus,Wistar rats were divided into high- dose group（STZ, 65 mg/kg, n= 12） and moderate-dose group（STZ, 55 mg/kg, n= 12）. Compared with Wistar rats, SD rats were injected intraperitoneally with moderate dose STZ （STZ, 55 mg/kg,n= 12）. SD rats were divided into two groups. The rats in one group（n-16） were in a single injection,in an other group （n=16） were injected twice （55 mg/kg on the first day and 55 mg/kg on the eighth day）,the OGTT was detected once in observation periods. The incidence and mortality of diabetes model were measured. Resuits：Showed that the incidence and mortality of Wistar rats were higher than that of SD rats Injecting twice could increase the incidence of diabetes rats and reduce the mortality The positive rate of abnormal OGTT was significantly higher than that of the positive rate of abnormal limosis blood glucose in the diabetic model rats whose pancreas had definite pathologic change. In conclusion, the animal diabetes model induced by streptozocin is steady and reliable, the incidence and mortality of diabetes model of Wistar rats were higher than that of SD rats. Using SD rats,moderate dose STZ and injecting twice that could increase the incidence of diabetes model and reduce the mortality. During animal experiment, OGTT has a better value than limosis blood glucose for making an estimate of the incidence of diabetes mellitus. Using OGTT can avoid missing diagnosis.