补阳还五汤对球囊扩张主动脉后再狭窄其内皮PDGFR和SOD-1基因表达的探讨

Study on Platelet-Derived Growth Factor mRNA and Copper-Zinc Superoxide Dismutase mRNA Expression Changes of Aortic Artery Endothelium with Restenosis after Aortic Artery Dilatation Treated with Buyang Huanwu Decoction

目的：探讨补阳还五汤防治经皮腔内冠脉成形术（ＰＴＣＡ）后再狭窄的消长机制。方法：仿ＰＴＣＡ术后再狭窄的新西兰兔模型２８只，以原位引物标记法测动脉内皮钢－锌超氧化物歧化酶（ＳＯＤ－１）及血小板行化生长因子受体（ＰＤＧＦＲ）的基因表达，分析再狭窄消长机理。结果：主动脉球囊扩张术后７天，手术部位动脉内皮的ＳＯＤ－１基因表达增强，与对照组有显著差异（Ｐ＜０．０５），而ＰＤＧＦＲｍＲＮＡ的表达则减弱，与对照组也有显著性差异（Ｐ＜０．０５）。结论：补阳还五汤增强动脉内皮ＳＯＤ－１的基因表达，改善缺血再灌注的供氧。同样情况下，补阳还五汤抑制ＰＤＧＦＲｍＲＮＡ表达，以控制细胞的病理性增殖。

Objective: To study on dynamic mechanism of restenosis after PTCA treated with Buyang Huanwu Decoction (BYHWD). Methods: Twenty-eight male New Zealand rabbits with mimic restenosis after aortic artery dilatation were detected by prime in situ labeling in aortic endothelium. The items included gene expression of copper-zinc superoxide dismutase (SOD-1 ) and platelet-derived growth factor receptor (PDGFR) mRNA to analyse dynamic mechanism of restenosis. Results: Seven days after aortic artery dilatation, PDGFR mRNA of aortic endothelium decreased clearly. The comparison between experimental and the control group showed significant difference (P <0. 05). By the same condition, the gene expression of SOD-1 was increasing, which showed a significant difference with the control group (P < 0. 05). Conclusions: BYHWD increased SOD-1 gene expression of artery endothelium,to improve oxygen feed for ischemia reperfusion in the same way, BYHWD suppressed PDGFR mRNA expression to control cellular pathological proliferation.