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抑制性消减杂交技术在对虾基因克隆中的应用 被引量：2

Application of Suppression Subtractive Hybridization in Shrimp Gene Cloning
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摘要 抑制性消减杂交技术是一项筛选、分离未知差异表达基因的试验方法,该技术具有快速、简便、灵敏、特异、高效,所需起始样本量较少等优点。利用该技术构建对虾组织消减cDNA文库,鉴定差异表达相关基因及其表达特性。通过对对虾差异性表达的免疫相关基因、抗病基因及其他性状基因的了解,旨在为进一步研究对虾抗病机理奠定基础,为利用抗病基因进行抗病育种提供一个有效途径,为提高对虾的经济效益提供前景。 Abstract：Suppression subtractive hybridization（SSH） technology is an experimental method that sieves and separates unknown differentially expressed genes. It possess some merits of speediness, brief, conven- ience, sensitivity, speciality, effectiveness and less quantity of the original sample, and so on. SSH was used to construct subtraetive eDNA libraries by suppression subtractive hybridization, which was em- ployed to identify differentially expressed genes and their expression profiles in shrimp tissue. Research on differentially expressed immune-relevant genes, virus-resistant genes and other genes, in order to build good base through further studying antiviral mechanism in shrimp, offers an effective channel for antiviral breeding through antiviral genes,and offers a prospect for raising economic effectiveness.
作者 赵永贞 蒋伟明 陈秀荔 李咏梅 张彦明 黎铭 陈晓汉
机构地区 广西水产研究所广西水产遗传育种与健康养殖重点实验室 西北农林科技大学动物医学院
出处 《动物医学进展》 CSCD 2008年第4期76-80,共5页 Progress In Veterinary Medicine
基金 广西科学研究与技术开发计划(桂科基0731034)
关键词 抑制性消减杂交 对虾 差异表达基因 CDNA文库 suppression subtractive hybridization shrimp differential expressed gene eDNA libraries
分类号 Q78 [生物学—分子生物学] S945.46 [农业科学—水产养殖]
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参考文献20

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共引文献4

同被引文献17

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引证文献2

二级引证文献7

动物医学进展
2008年 第4期

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