摘要
针对冬季低温(<17℃)条件下,如何以优良厌氧发酵菌种和沼气促进剂提高农村户用沼气池产气量,进行了研究。研究中以特制的5L厌氧消化罐作反应器,以猪粪为底物,设计了Fe3+,Ni2+,木炭3因素4水平的正交实验,研究厌氧发酵过程中产气量的变化,以获得微量元素和木炭的最佳的投加量组合配比,在此基础上驯化出优良的产甲烷菌种。沼气促进剂和菌种的实验室和农户应用效果表明,在厌氧发酵过程中微量元素和木炭都能够增强甲烷菌活性,提高产气量,并对低温具有一定的抵抗作用。其最佳配比Fe3+:Ni2+:木炭为2:1:5,投加绝对量为0.61%(相对于原料干重)。驯化出的菌种微生物生物量较高,较驯化前提高了27.3%,作为接种物在农村沼气实际运用中取得较好效果。
This study was to explore effective ways to increase biogas production of rural household with good anaerobic fermentation bacteria and methane accelerator in lower temperature （ 〈 17℃ ） in winter. Fe^3＋ , Ni^2＋ , and charcoal, three factors-four level orthogonal experiment was designed with special 5 L anaerobic digestion tank as the reactor and pig dung as the substrate. Biogas production in the anaerobic fermentation process was studied to get the best dosage mix ratio of trace clements and charcoal. On the basis of these, good methanogen was domesticated. The result showed that in the process of anaerobic fermentation both trace elements and charcoal can enhance the activity of methane bacteria, increase gas production, and resist low temperatures. The best ratio of Fe^3＋ ： Ni^2＋ ： charcoal was 2： 1： 5, and absolute volume of 0. 61% （compared with dry weight of raw materials）. Microbial biomass of domestication bacteria was 27.3% higher than without domestication. It had good effects in the actual use of biogas in rural household as inoculum.
出处
《能源工程》
2008年第2期31-35,共5页
Energy Engineering
关键词
微量元素
驯化
甲烷菌
微生物生物量
trace element
domesticates
methane bacteria
microbial biomass
