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急性脑静脉闭塞动物模型的建立及磁共振评价

Establishment of acute cerebral venous occlusion and evaluation of it with magnetic resonance imaging
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摘要 目的:拟建立一种稳定的急性脑静脉闭塞动物模型,用磁共振DWI、PWI、MRS、DTI对模型进行评价,观察脑实质损害的变化规律。方法:家猫30只,随机分为2组(手术组24只,假手术组6只),手术组采用开颅上矢状窦穿刺注射醋酸纤维素聚合物(Cellulose acetate polymer,CAP)制备急性脑静脉闭塞模型,假手术组仅行开颅上矢状窦暴露,术后1、3、6、12、24、48h对各组模型行MRI检查。结果:手术组22只猫造模成功。DWI发现14只猫脑实质出现25个异常信号,PWI发现17只猫脑实质内31个异常灌注区。MRS检查示病变区Lac术后1h升高明显,以后逐渐降低,术后3h病变区NAA开始降低。DTI检查示术后1h,ADCav值降低,3h后逐渐增高,FA值于术后各时间点均降低。病理学检查见病灶以血管源性水肿为主,12h后出现静脉性脑梗塞、脑出血。假手术组脑实质未见异常改变。结论:上矢状窦穿刺注射CAP制备急性脑静脉闭塞模型方法可行,该模型适合于病理生理及影像学研究。DWI结合PWI、MRS、DTI对急性脑静脉闭塞脑实质损害的动态变化、发病机理的判断具有重要价值。 Objective:To establish a stable animal model of acute cerebral venous occlusion and to evaluate the model with DWI,PWI,MRS and DTI in observing the changing regularity of the brain parenchymal lesions. Methods:Thlrty cats were randomly divided into 2 groups,operation group(n=24) and sham operation group(n=6). Operation group was performed by injection of the cellulose acetate polymer(CAP) solution into the superior sagittal sinus(SSS) to establish the acute cerebral venous occlusion,while sham operation group underwent only the exposure of SSS. MRI was performed at 1,3,6,12,24 and 48h after operation. Results:Twenty-two cats in operation group were operated successfully. Twenty-five lesions were detected on DWI in 14 cats. Thirty-one abnormal perfusion regions were detected on PWI in 17 cats. MRS showed that Lac increased obviously in lh after operation and decreased gradually later,and NAA decreased in 3h. DTI showed that ADCav values decreased in lh,increased 3h later and FA values decreased at all time points after operation. The microscopic changes were mainly vasogenic edema and followed by venous cerebral infarction and cerebral hemorrhage in 12h. No abnormal findings were observed in sham operation group. Conclusion:Establishment of acute cerebral venous occlusion model by injection of CAP solution into SSS is feasible. The model is suitable for pathophysiological and radiological studies. DWI combined PWI,MRS and DTI have great value in judging the dynamic changes and the etiopathogenesis of brain parenchymal lesions in acute cerebral venous occlusion.
出处 《重庆医科大学学报》 CAS CSCD 2008年第4期481-485,496,共6页 Journal of Chongqing Medical University
关键词 模型 脑静脉闭塞 磁共振成像 Model Cerebral venous occlusion Magnetic resonance imaging
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参考文献12

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