以RT-PCR技术扩增并鉴定小鼠I-Eβ基因及其意义

AMPLIFICATION AND VERIFICATION OF I-Eβ GENE OF MUS MUSCULUS THROUGH RT-PCR AND SIGNIFICANCE

目的:以RT-PCR方法体外扩增小鼠I-Eβ基因并对其进行鉴定。方法:以Trizol试剂提取近交系BLAB/c小鼠血液及脾脏总RNA,以RT-PCR方法进行扩增得到I-Eβ基因,并进行测序鉴定。结果:提取RNA片段完整,纯度高;以RT-PCR扩增得特异性I-Eβ片段;测序结果与Genebank上该片段序列一致。结论:小鼠I-Eβ基因是一个中-高转录基因,其分离和鉴定是许多后续研究的前提。

Objective：To amplif and verify I-Eβ gene of mus musculus through RT-PCR. Methods： Total RNA was extracted by Trizol reagent from the blood and spleen tissue of BLAB/c mus musculus which is inbred; I-Eβ gene was amplified in vitro through RT-PCR, and verified by detecting its array from PCR production.Results： The fragment of total RNA was integrate, and purity was well. The specificit I-Eβ fragment is amplified by RT-PCR. The array was tallied with the fragment in Genebank.Conclusion：Amplifying and verifying I-Eβ of Mus musculus is the foundation for further researchs.