四君子汤加味大黄对烫伤大鼠免疫功能的影响

EFFECT OF SIJUNZI DECOCTION PLUS RHUBARB ON IMMUNE FUNCTION IN RATS AFTER BURN

目的:观察早期口服四君子汤加味大黄对烫伤大鼠外周血T细胞亚群和IgA以及肠道分泌S-IgA的影响。方法:复制大鼠烫伤模型,随机分为正常对照组、烫伤中药治疗组、烫伤对照组,用APAAP法测定伤后各时相点外周血CD4+、CD8+细胞数量并计算CD4+/CD8+比值;用速率散射比浊法测定伤后各时相点外周血IgA;用放射免疫分析法检测伤后各时相点回肠粘液中S-IgA的含量。结果:烫伤对照组外周血CD4+细胞数量明显减少,CD8+细胞数量增多,CD4+/CD8+比值明显降低,回肠粘液中S-IgA以及血浆IgA均明显降低,且与正常对照组有显著差异,而烫伤中药治疗组6-9天外周血CD4+细胞数量逐渐增加,CD8+细胞数量逐渐减少,CD4+/CD8+比值逐渐升高,回肠粘液中S-IgA及血浆IgA均明显高于烫伤对照组,且与正常对照组无明显差别。结论:烧伤后T淋巴细胞数量严重紊乱,血浆IgA及回肠粘液中S-IgA亦有降低,提示早期口服中药四君子汤加味大黄可明显提高烧伤大鼠外周血CD4+细胞数量,降低CD8+细胞数量,增加CD4+/CD8+比值,使烧伤后T细胞数量恢复正常,并有效维持肠粘膜细胞分泌S-IgA的功能。

Objective： To observe the effect of early oral feeding with SiJunZi decotion plus rhubarb on T cells subgroup and IgA in peripherial blood,and the concentration of S-IgA in the ileal mucus in rats after burn. Methods： Rats post-burn were randomly divided into three groups： normal control group, burned control group, and treatment group.The numbers of T cells （CD4^＋、CD8^＋） in peripherial blood were measured by APAAP, the plasma IgA content was measured by immune turbidity degree, the S-IgA concentration of ileal mucus was measured by RIA. Results： The number of CD4^＋ T cell and CD4^＋/CD8^＋ Ratio, the concentration of S-IgA in the ileal mucus as well as IgA in plasma decreased significantly post-burn in burned control group, but increased in treatment group with SiJunZi decoction 6-9 days post-burn, the number of CD8^＋ T cell decreased conversely. Conclusion： The results indicated that the treatment with SiJunZi decoction plus rhubarb in post-burn could efficiently raise the number of CD4^＋T cell,CD4^＋/CD8^＋ Ratio in peripherial blood and the plasma IgA content, efficiently improve the small intestinal S-IgA excretion.