血清一氧化氮和IL-10水平与肝硬化患者病情变化关系探讨

INVESTIGATION OF THE CORRELATION BETWEEN SERA NO AND IL-10 LEVELS AND THE STATE OF HEPATIC CTRRHOSIS

目的:探讨血清中一氧化氮(NO)和白介素-10(IL-10)水平的变化与肝硬化病情演变过程的相互关系及其可能的作用机制。方法:应用硝酸还原酶法和ELISA法测定72例活动期和恢复期肝硬化患者,76例活动期和恢复期慢性肝炎患者,76例活动期和恢复期急性肝炎患者及60例正常人血清中NO和IL-10水平的交化。结果:肝硬化患者血清中NO水平与其他三组比较显著升高,恢复期患者也不能降至正常水平(P<0.01),而IL-10水平明显低于对照组(P<0.01)。活动期的慢性肝炎患者和急性肝炎患者血清中NO水平与正常组比较有不同程度的升高(P<0.01),但不如肝硬化组患者显著(P<0.01)。急性肝炎患者恢复期血清中NO水平可降至正常水平。慢性肝炎患者和急性肝炎患者活动期血清中IL-10水平也表现为不同程度的降低。相关性分析结果显示:肝硬化患者血清中NO水平与IL-10水平呈明显负相关(r=-0.469,P<0.01)。结论:IL-10和NO参与肝硬化病情演变机制,并可作为检测病情演变的可靠指标之一。

Objective：To investigate the changes of sera NO and IL-10 in patients with hepatic cirrhosis and their correlation with the state of illness.Methods：Sera from 72 patients with active and convalescent hepatic cirrhosis,76 patients with active and convalescent chronic hepatitis,76 patients with active and convalecent acute hepatitis and 60 normal controls were tested for NO and IL-10 with ELISA assay.Results：Compared with the other three groups,level of NO in patients with hepatic cirrhosis was significantly elevated,nd was not able to decrease to normal level even in convalescent phase（P〈0.01）;IL-10 level in patients with hepatic cirrhosis decreased significantly （P〈0.01）.Levels of NO in patients with active acute or chronic hepatitis were also elevated,but were not as high as that in patients with hepatic cirrhosis（P〈0.01）.NO in patients with acute hepatitis in its convalescent phase was able to be at normal level.IL-10 level in patients with active chronic and acute hepatitis decreased.Correlation analysis data showed that levels of NO correlated negatively with IL-10 in patients with hepatic cirrhosis （r=- 0.496,P〈0.01）.Conclusion：NO and IL-10 were involved in the formation and developing of hepatic cirrhosis,and the detection of NO and IL-10 may be one of the reliable methods in evaluation of patient＇s condition.