高压氧对大鼠肢体缺血再灌注致腹部器官损伤的作用

Rat Abdominal Organ Dysfunction Caused by Rat Hind Limb Ischemia/reperfusion and the Effect of Hyperbaric Oxygen

目的探讨高压氧(HBO)对减轻大鼠肢体缺血再灌注(I/R)致腹部器官损伤的作用及其机制。方法复制缺血2h再灌注1h动物模型并分为3组,即A组(假手术组)、B组(I/R模型组)、C组(HBO治疗组)。观察实验动物肝、胰、肾及回肠微血流变化;记录血清血小板膜糖蛋白CD31、CD61平均荧光强度和CD62p的阳性率。结果与B组比较,C组肝脏及肾脏微血流速度明显升高,差异有统计学意义(P<0.05)。与B组比较,C组上述各脏器组织病理改变在一定程度上有缓解。与A组比较,B组CD31、CD61及CD62p均升高,其中CD31和CD62p升高明显,差异有统计学意义(P<0.05);与B组比较,C组CD31、CD61及CD62p均有下降趋势,其中CD31和CD62p下降尤为明显,差异有统计学意义(P<0.05)。结论早期行HBO治疗可显著减少急性下肢I/R大鼠模型血小板膜糖蛋白CD31和CD62p的表达,有助于减轻下肢I/R继发腹部器官损伤。

Objective To determine whether there is a potential protective effect of HBO on abdominal organ injury which is associated with hind limb ischemia/reperfusion in rat model. Methods Adult male SD rats weighing 220 - 250 g were used in the study. Bilateral hind limb ischemia was induced by applying an atraumatic clip at the end of the abdominal aorta adjacent to the junction of both femoral arteries. At the end of the ischemic period, reperfusion of the limbs was achieved by releasing the clamp. The experimental animals were randomly divided into three groups, each group involved 10 rats. （1） the sham surgical group; （2） the ischemia/ reperfusion（I/R） group; （3） the hyperbaric oxygen（HBO） treatment group. By using laser Doppler flowmetry, the microvascular blood flow in the liver, kidney, pancreas and small intestine of animals was measured. The mean immunofluorescence intensity of CD31, CD61 or positive percentage of CD62p were measured by the flow cytometry. Results Compared with I/R group, the microvascular blood flow of the liver and kidney in HBO treatment group was significantly increased, with a significant statistical difference （ P 〈 0.05 ）. But the microvascular blood flow of pancreas and small intestine were not obviously changed in these three groups. In the I/R group, extensive ultrastructural histological injury of liver and kidney occurred. The uhrastructural pathological changes in the HBO treatment group were obviously alleviated. Compared with the sham surgical group, the expression of platelet membrane glycoprotein CD31, D62p in the I/R group was much higher（P 〈0.05）. The expression of CD31, D62p in the HBO treatment group was much decreased than that in the I/R group（P 〈 0.05 ）. Conclusion As a kind of effective therapy, HBO can alleviate the pathological changes in abdominal organs in rat models caused by hind limb ischemia/reperfusion.