过氧化物酶体增生物激活受体γ基因Pro12Ala多态性与高血压的相关性研究

Association of Peroxisome Proliferator Activited Receptor-γ Gene Pro12Ala Polymorphism and Hypertension

目的研究过氧化物酶体增生物激活受体γ基因Pro12Ala多态性与高血压的相关性。方法对2003年10月至2005年10月在首都医科大学宣武医院心血管内科就诊的北京地区汉族人群中高血压病患者583例及同期健康查体的正常对照者363例进行病例-对照研究,采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP),检测2组受试者过氧化物酶体增生物激活受体γ基因Pro12Ala多态性的基因型及等位基因频率,比较2组基因型和等位基因频率分布的差异,并分析不同年龄、性别间高血压组与正常对照组基因型及等位基因频率的差异。同时分析各组不同基因型间血压的差异。结果946例研究对象等位基因型及基因型频率的分布分析结果显示,PP基因型者占90.91%,PA基因型占8.98%,AA基因型占0.01%,P等位基因频率占95.40%,A等位基因频率占4.60%。其中高血压组583例受试者中PP、PA和AA基因型分别为533(91.42%)、49(8.40%)和1(0.18%);正常对照组受试者中PP、PA和AA基因型分别为327(90.08%)、36(9.92%)和0(0.00%)。χ2检验2组基因型及等位基因频率分布显示差异无统计学意义,2组不同基因型间血压差异也没有统计学意义(P>0.05)。进一步按照性别分组后,高血压组与正常对照组不同性别受试者基因型及等位基因频率分布差异无统计学意义,不同性别基因型间血压差异亦无统计学意义。按照年龄分组后(≥60岁与<60岁组),不同年龄高血压组与正常对照组基因型与等位基因间差异无统计学意义,2个年龄组各基因型间血压差异亦无统计学意义。结论过氧化物酶体增生物激活受体γ基因Pro12Ala多态性可能与高血压无明显相关性。

Objective To test the association of Pro12Ala polymorphism of peroxisome proliferator activated receptor-γ gene in exon 2 with hypertension. Methods Five hundred and eighty-three hypertensive and 363 corresponding normal controls in Beijing Chinese Han population who came to hypertensive clinic for treatment or check-up from October 2003 to October 2005 in Xuanwu hospital were involved in the research. PCR-RFLP method was used to determine the distributions of A allele and PP, PA, AA genotype frequencies of Pro12Ala polymorphisms of peroxisome proliferator activated receptor-γgene in exon 2. Parameters that satisfied normal distribution were expressed as mean ± standard deviation （ SD ）, abnormal distribution data were expressed as median and quartile. Statistical analysis was performed using SPSS version 11.5. The Hardy-Weinberg equilibrium was performed using the χ^2 test. Student t-test. rank sum test were used to comoare differences between genotype groups. Pearson＇s χ^2 test was used to compare the genotype prevalence between different groups. P values 〈 0.05 were considered statistically significant. At the same time subjects were divided into different groups by gender and age for further analysis. The SBP, DBP, MAP level between different genotypes in different groups were also analyzed. Results Of the 946 Beijing Chinese Han subjects, Pro12Pro genotype was present in 90.91% （860 subjects） of the population, Pro12Ala genotype was present in 8.98% （85 subjects） of the population and for Ala12Ala genotype 0.01% （ 1 subject） of the population. Allele frequencies were 95.40% for Pro allele and 4.60% for Ala allele. In hypertensive group, the frequencies of PP, PA, AA genotype and A allele of peroxisome proliferator activated receptor-γ gene were 533 （91.42%）, 49 （ 8.40% ）, 1 （ 0. 18% ） respectively. In control group, the frequencies of PP, PA, AA genotypes and A allele were 327（90.08% ）, 36（9.92% ）, 0（0.00% ） respectively. Analyzed by χ^2 test, the distribution of PP, PA, AA genotype and A allele frequency were not different between hypertensive group and normal controls （P 〉 0.05）. There was no significant difference in SBP, DBP, MAP between PP and PA-AA genotypes. When the subjects were divided into two groups by genders, the distribution of PP, PA, AA genotypes and A allele was of no statistical difference. There was also no significant difference in SBP, DBP, MAP between PP and PA-AA genotypes in different genders. Analyzed by age, the subjects were divided into two groups（less than sixty, sixty and above）, we compared the frequencies of PP, PA, AA genotypes and A allele between hypertensive and normal controls in different groups, no statistically difference had been found. At the same time, the SBP, DBP, MAP level between PP and PA-AA genotypes in different aged hypertensive and their corresponding normal controls did not reach significantly statistical difference. Conclusion The results suggest that Pro12Ala polymorphisms in peroxisome proliferator activated receptor-γ gene may not be associated with hypertension.