血清中硝酸盐的示波极谱测定法研究

STUDY ON THE ANALYTICAL METHOD OF NO_3^-IN SERUM BY OSCILLOPOLAROGRAPHY.

取血清０．１０ｍｌ，加入０．６０ｍｌ饱和硫酸铵和０．３０ｍｌ饱和硫酸银以沉淀蛋白质和氯离子，然后离心除去。在浓硫酸介质中，ＮＯ－３与麝香草酚反应生成硝基酚化合物，在氨碱性条件下，用示波极谱法测定。方法检出下限为４．８μｇ／Ｌ，相对标准差为３．３％—６．３％，回收率为９５．７％—１１３．０％（ｘ＝１０４．２％），用该法测定了２８例心脏病患者血清中的ＮＯ－３含量，结果为７４．０±１２．５μｍｏｌ／Ｌ。

In 010ml of serum sample,060ml saturated(NH_4)_2SO_4 and 030ml of saturated Ag_2SO_4 are added to precipitate the proteins and Cl^-,and then centrifugalize to remove the precipitates.In the concentrated H_2SO_4 medium,NO_3^- reacts with thymol to form a nitrophenolic compound which is determined by oscillopolarography in ammonia alkaline medium.The method has a detection limit of 48μg/L and the RSDs of 33%～63%.The recoveries ranged from 957% to 1130% with an average recovery of 1042%.The nitrate concentrations of 28 serum samples from the patients with heart diseases have been determined by this method with the results of 740±125μmol/L.