ET-1神经分布异常与高血压鼠脑动脉神经源性调节的关系

RELATIONSHIP BETWEEN ABNORMAL ENDOTHELIN-1 INNERVATION AND NEUROGENIC REGULATION OF CEREBRAL ARTERIES IN HYPERTENSIVE RATS

目的探讨ET-1(Endothelin-1,ET-1)能神经纤维分布与高血压鼠脑血管的神经源性调节的关系,探讨ET-1神经是否参与高血压时期脑血流的调节。方法应用免疫组织化学技术观察自发性高血压鼠和Wistar正常血压鼠脑底动脉(包括大脑前动脉、大脑中动脉、大脑后动脉和基底动脉)ET-1能神经纤维的分布密度和走行方式。结果自发性高血压鼠和Wistar正常血压鼠脑底动脉均可见棕褐色的ET-1能免疫反应阳性纤维,似细线状,攀附于血管壁上,自发性高血压鼠脑底动脉各主要分支ET-1能免疫反应阳性纤维密度较Wistar正常血压鼠明显增加,纤维走行大多呈网状。结论实验结果提示自发性高血压鼠脑底动脉增加的ET-1能免疫反应阳性纤维可能与脑血管的神经源性调节有关;高密度的ET-1能神经纤维可能涉及高血压时期脑血流的调节。

Objective To investigate the relationship between endothelin-1 （ ET-1 ） perivascular nerves and the neurogenic regulation of cerebral vessels in spontaneously hypertensive rats （ SHRs）, and whether ET-1 participates in the regulation of cerebral blood flow during high blood pressure in SHR. Methods Immunohistochemical techniques were used to observe the ET-1 immunoreactive nerves on cerebral arteries, including anterior cerebral artery （ACA）, middle cerebral artery （ MCA）, posterior cerebral artery （PCA） and basilar artery （ BA）, in SHRs and normotensive Wistar rat controls. Results The area density of ET-1 perivascular nerves on cerebral arteries was denser in SHRs than in control. Conclusion The experimental results suggest that enhanced ET-1 perivascu- lar nerves on cerebral artery may play an important role in the neurogenic regulation of cerebral vessels in SHRs. The elevated ET-1 nerve fiber density of cerebral arteries may be involved in the regulation of the cerebral blood flow during pronounced arterial hypertension in SHRs.