向日葵耐盐突变体筛选

Screening of Sunflower Mutant with Salt-tolerant Ability

以MS为基本培养基,研究了不同激素对康地5号向日葵愈伤组织的诱导效果,筛选出了最佳诱导愈伤组织培养基;以子叶、下胚轴及真叶作为外植体,确定了子叶和下胚轴作为康地5号组织培养的最佳外植体;在含50、100、及150mmol/L NaCl不同盐浓度培养基上分别诱导出向日葵耐盐愈伤组织,继代培养后得到不同耐盐程度的新株系,移栽后得到完整植株.

The effects of different plant hormones on the sunflower callus induction were investigated with MS as the basic culture medium. Consequently , the best culture medium for callus induction was selected out . Compared with Cotyledons , euphyllas, and Hypocotyls , Cotyledons and Hypocotyls was the best explant for sunflower tissue culture. The callus of sunflower which was induced from the medium containing 50, 100, or 150mmol/L NaCl was successfully regenerated and transferred into the soil .