白藜芦醇对去卵巢大鼠股骨骨保护素及核因子κB受体活化子配体表达的影响

Effects of resveratrol on the expression of osteoprotegerin and receptor activator for NF-κB ligand in femurs of ovariectomized rats

目的:观察白藜芦醇对去卵巢大鼠股骨骨保护素(OPG)及核因子κB受体活化子配体(RANKL)表达的影响。方法:健康3月龄雌性SD大鼠48只,按体重随机分为6组:假手术组(SHAM)、单纯卵巢切除组(OVX)、17β-雌二醇组(ERT,0.1mg.kg-1·d-1)。低、中、高剂量白藜芦醇组(RL、RM、RH,每天分别给予10、20、40mg/kg白藜芦醇)。除假手术组外其余各组均切除双侧卵巢。术后1周开始给药,给药8周后处死所有大鼠,测定股骨骨密度(BMD)及骨生物力学性能:弹性模量(ELASTIC)、刚度(STIFFNESS)、最大应力(M-STRESS)及最大承载力(M-LORD)。用免疫组织化学染色方法观察股骨OPG、RANKL的表达。结果:与OVX组比较,20、40mg·kg-1.d-1白藜芦醇均能上调股骨OPG表达(P<0.05)。与OVX组比较,20、40mg.kg-1·d-1白藜芦醇均下调RANKL的表达,改善股骨骨密度及生物力学性能(P<0.05)。结论:白藜芦醇在体内可上调骨组织中OPG的表达,下调RANKL的表达,这可能是其改善股骨骨密度及生物力学性能的作用机制。

AIM： To study effects of resvemtrol on osteoprotegerin（OPG） and receptor activator of NF-κB ligand（RANKL） expression in femur of ovariectomized rats. METHODS： 48 female SD rats （3 months old） were assigned into 6 groups randomly： sham-operated group（SHAM） ; ovariectomized group（OVX） ; 17β-estradiol replacement therapy group （ ERT, 0.1 mg·kg^- 1·d^- 1, sc） ; high-dose resveratrol-treate group （RH, 40 mg·kg^- 1·d^- 1, i.p. ） ; middle-dose resveratrol-treated group （ RM, 20 mg·kg^- 1·d^- 1, i. p. ） ;low-dose resveratrol-treated group （ RL, 10 mg·kg^-1·d^-1, i. p. ）. Except for SHAM group, rats in other groups were operated by bilateral ovariectomy. Administration started at the 8th day after operation and lasted 8 weeks. At the end of the 9th week after operation, all rats were sacrificed. The bone mineral density （BMD） and vitodynamics properties（ including ELASTIC, M-STRESS, M-LORD and STIFFNESS） of femurs were measured. The expression of OPG and RANKL in femurs of rats were observed by immunohistochemical staining methods. RESULTS： Compared with OVX group, resveratrol treatment （ 40 mg·kg^-1·d^-1 and 20 mg·kg^-1·d^-1 ） increased expression of OPG in femurs while decreased expression of RANKL in ovariectmized rats. In RH, RM, and ERT group, BMD, ELASTIC, M-STRESS of femurs were higher than those of OVX group. CONCLUSION： Resveratrol increased expression of OPG in femur while decreased expression of RANKL in ovariectmized rats, which was probably one of mechanism that resveratrol improve osteoporosis.