不同抗高血压药物对自发性高血压大鼠左心室肥厚及心钠素mRNA表达的影响

Effect of Various Antihypertensive Drugs on Left Ventricular Hypertrophy in Relation to the Expression of Atrial Natriuretic Peptide mRNA in Left Ventricle in Spontaneously Hypertensive Rat

探讨抗高血压药物对自发性高血压大鼠（ＳＨＲ）左心室肥厚与心钠素（ＡＮＰ）ｍＲＮＡ表达的影响。方法成年（１６周龄）雄性ＳＨＲ测定收缩压后随机分为３组，分别给予卡托普利（１００ｍｇ／ｋｇ·ｄ－１），双氢克尿塞（１００ｍｇ／ｋｇ·ｄ－１），普萘洛尔（４０ｍｇ／ｋｇ·ｄ－１）治疗，共１２周，年龄、性别及数量配对的正常血压的ＷＫＹ及未治疗ＳＨＲ作为对照组。治疗结束时测体重、收缩压。动物断头处死，取左心室称重后，采用异硫氰酸胍一步法提取组织总ＲＮＡ，Ｎｏｒｔｈｅｒｎ杂交法检测ＡＮＰｍＲＮＡ相对表达量。结果治疗组动物收缩压及左心室重／体重比均明显低于未治疗ＳＨＲ组。卡托普利与普萘洛尔明显抑制心钠素基因的表达，双氢克尿塞对心钠素基因的表达影响较小。结论不同抗高血压治疗在降低血压和逆转左心室肥厚的同时也不同程度地抑制左心室心钠素基因的表达，利尿剂对心钠素表达的影响较小。

Aim To investigate the effects of various antihypertensive drugs on left ventricular hypertrophy and the expression of atrial natriuretic peptide(ANP) in the left ventricle in spontaneously hypertensive rat(SHR).\ Methods After the measurement of systolic blood pressure(SBP), adult male SHRs(16 wk old) were randomly divided into 3 groups. Ratseach was given captopril(Cap,100 mg/kg·d -1 ),dihydrochlorothiazide(DCT,100 mg/kg·d -1 ),propanolol(Pro,40 mg/kg·d -1 ) for 12 weeks respectively ,sex age and number matched untreated SHR and normotensive WKY served as controls.\ Body weights and SBP were measured before decapitation.\ Left ventricle was subjected to guanidine thiocyanate phenol chloroform procedure for RNA extraction.\ Left ventricular hypertrophy(LVH) was assessed by left ventricle weight to body weight ratio(LVW/BW).\ ANP expression was deternmined by Northern Blot.\ Results Treated animals in Cap and pro groups showed a significant lowering in SBP and LVW/BW, while LVW/BW in DCT treated SHR was similar to that of WKY(3 29±0 33 vs 2 80±0 64 mg/g,P>0 05).\ The expression of ANP mRNA was supressed by the three drugs. The decreased degree of ANP was more slight in DCT group compared with cap and pro groups.\ Conclusion Accompanied by the reversion of LVH, expression of ANP was supressed with various degree by different antihypertensive drugs.\ Difference in supressive effects of various drugs on ANP expression may be thhe results of the different hemodynamics and cardiovasoactive factors in hypertension.