摘要
目的:建立一种测定中成药锁阳固精丸中掺入化学药枸橼酸西地那非的检测方法。方法:采用高效液相色谱法,色谱柱C18(250mm×4.6mm,5μm),0.05mol/L磷酸三乙胺溶液-甲醇-乙腈(58∶25∶17)为流动相;检测波长为290nm,流速为1.0ml/min。结果:测得枸橼酸西地那非在18.5~166.5μg/ml浓度范围内线性关系良好,平均回收率为99.38%,RSD=0.62%。结论:本法操作简便,结果可靠,可用于锁阳固精丸中非法添加物枸橼酸西地那非的检测。
Objective:To set up a practical method for determination of sildenafil citrate in Suoyang Gujing Pills.Methods:The C18 column(250 mm×4.6 mm,5 μm)was used,mobile phase was 0.05 mol/L potassium triethylamine-methanol-acetonitrile(58∶25∶17),the detective wavelength was set at 290 nm,and the flow rate was 1.0 ml/min.Results:The liner range of sildenafil citrate was 18.5~166.5 μg/ml.The average recovery was 99.38% with RSD of 0.62%.Conclusion:The method is simple,accurate.It can be used to determine sildenafil citrate in Suoyang Gujing Pills.
出处
《中国医药导报》
CAS
2008年第11期50-51,共2页
China Medical Herald