改性硼硅酸盐生物玻璃的可控降解性能(英文)

Controllable degradation of a novel borosilicate glass for bone tissue engineering

背景:生物玻璃和生物陶瓷由于良好的生物相容性和生物活性得到了更多的关注。但其材降解性较低限制了在骨组织工程方面的应用。目的:观察硼硅酸盐生物玻璃改性后可控降解性变化。设计:单一样本观察。单位:同济大学材料科学与工程学院。材料:实验于2005-10/2007-09在同济大学材料科学与工程学院完成。实验用分析纯的碳酸盐、磷酸盐、硼酸及二氧化硅为自制品,D/max2550VB3+/PCX线衍射仪及S-2360扫描电镜均为日本Olympus公司生产。方法:以硼硅酸盐玻璃粉为原料,采用有机泡沫浸渍工艺,制备高孔隙率的网眼多孔支架。玻璃材料组成25Na2O·30CaO·5P2O5·(40-x)SiO2·xB2O3中,x分别取0,20,26,30及40作为不同构成比。主要观察指标:①应用X线衍射仪、扫描电镜观察各组分材料表面羟基磷灰石形成情况及支架形貌。②原子发射光谱检测材料在K2HPO4溶液中钠、钙离子、磷和硼离子的浓度。结果:①25Na2O·30CaO·5P2O5·(40-26)SiO2·26B2O3组分的硼硅酸盐生物玻璃具有更好的孔隙连接性,孔隙结构可满足细胞于支架上的增殖。②25Na2O·30CaO·5P2O5·40SiO2组分的硼硅酸盐生物玻璃中钠及硼离子释放较低,降解速率低于其他组分。结论:通过调整玻璃的组成,可控制材料的降解性和表面形成羟基磷灰石晶体的形态。

BACKGROUND： With excellent biocompatibility and bioactivity, bioglass and bioceramics have attracted more attention. However, the poor degradability limits their application in bone tissue engineering. OBJECTIVE： To observe the bioactivity and controllable degradation of a novel borosilicate glass. DESIGN： Single sample observation. SETTING： School of Materials Science and Engineering, Tongji University. MATERIALS： The experiment was performed at School of Materials Science and Engineering, Tongji University from October 2005 to September 2007. Thee reagent grade chemicals such as carbonate, phosphate, boric acid and silicon dioxide were self-made; D/max2550VB3＋/PC X-ray diffractometer and S-2360 scanning electron microscope （SEM） were products of Olympus, Japan. METHODS： Three-dimensional, highly porous scaffolds with interconnected pores similar to trabecular bone were fabricated using melt-derived borosilicate glass powder by polymeric sponge method. The composition of glass material was 25Na/O ·30CaO · 5P2O5· （40-x）SiO2· xB2O3 （x=0, 20, 26, 30, 40）. MAIN OUTCOME MEASURES： ①Hydroxyapatite formation on the surfaces of the samples and morphologies of the scaffolds were observed by X-ray diffractometer and SEM. ②Ion concentrations of the Na^＋, Ca^2＋, p^5＋ and B^3＋ in the solution of K/HPO4 were determined using atomic emission spectrum. RESULTS： ①The borosilicate glass made of 25Na2O· 30CaO· 5P2O5·（40-26）SiO2·26B2O3 showed better pore connectivity, and cells proliferated on the porous structure.② In the borosilicate glass made of 25Na10·30CaO·5P105 ·40SiO2, Na^＋ and B^3＋ release was less and their degradability was lower than other compositions. CONCLUSION： Hydroxyapatite formation on the surface and the degradation rate of scaffold materials could be controlled by changing the composition of the bioglass samples.