HPLC-ELSD内标法测定知母药材中菝葜皂苷元含量

Determination of sarsasapogenin in Rhizoma Anemarrhenae by HPLC-ELSD internal standard method

目的:建立 HPLC—ELSD 内标法,测定知母药材中菝葜皂苷元含量。方法:采用胆同醇为内标,Kromasil C_(18)柱(250mm×4.6mm,5μm),以甲醇为流动相,流速1 mL·min^(-1)。柱温为30℃。蒸发光散射检测器漂移管温度为50℃,蒸发温度为70℃,以氮气为雾化气,压力为1.03×10~5Pa。结果:菝葜皂苷元进样浓度存0.02～0.50 mg·mL^(-1)内,进样量的常用对数与对照品峰和内标峰面积比值的常用对数成良好线性关系(r=0.9995);平均回收率(n=5)为96.3%,RSD 为2.1%。结论:建立的内标方法准确、快速,是控制知母药材质量较理想的方法。对我国北方主要知母产地药材中菝葜皂苷元含量测定和比较表明不同产地野生知母质量差异较大。

Objective：To establish an HPLC - ELSD internal standard method for determination of sarsasapogenin in Rhizoma Anemarrhenae. Method： With cholesterol as internal standard , Kromasil C18 column （250mm×4.6mm,5μm）was used . Mobile phase was methanol with the flow rate of 1 mL·min^-1;the temperature of the drift tube and evaporation was 50℃ and 70℃ respectively. The gas pressure was 1.03×10^5 Pa. Results： There was good linearity in the range 0.02 - 0.50μg·mL^-1 of sarsasapogenin r = 0.9995 ;the average recovery was 96.3 % with RSD of 2. 1%. Conclusion ： The internal standard method is rapid and accurate. It is suitable for quality control of Rhizoma Anemarrhenae. The result of determination reveals that the quality of Rhizoma Anemarrhenae from different places of north China are of notable difference.