普鲁卡因等27种青霉素及头孢菌类的~1H、^(13)C-NMR谱解析规律

The spectrum rules of ~1H、^(13)CNMR on the twenty-seven kinds of procaine benzylpenicillin and cephalosporin compounds

目的:总结普鲁卡因等27种青霉素及头孢菌类的核磁共振氢、碳谱规律,解析青霉素与头孢菌类的分子结构的异同和归属。方法:核磁共振一维氢、碳谱,采用室温、常规测试方法。结果:青霉素与头孢菌类具有~1H—NMR 差异特征峰,青霉素类分子中,四圆环上的二个-CH 多数表现为单峰且重叠,显示 A_2系统。头孢菌类分子中,四圆环上的二个-CH 均表现为双峰,且不重叠,显示 AB 系统。在^(13)C—NMR 谱中两类谱图无差异,该-CH 峰不重叠。结论:核磁共振一维氢谱,简便、快速、灵敏,为药物结构的鉴定提供有效可靠的方法。

Objective： The Spectrum Rules of twenty -seven procaine benzylpenicillin and cephalosporin compounds are summarized and their molecular structures are confirmed by ^1H,^13CNMR. Methods：^1H,^13CNMR Spec-trum, room temperature and general tests. Results： The ^1HNMR shows both of penicillin and cephalosporin compounds have different characterltic peaks. The two -CH groups in four- ring in the most of penicillin compounds show overlapped single peak, but the two- CH groups in cephalospofin compounds show no overlapped double peaks,which indicates A2 system in the penicillin compounds and AB system in the cephalosporin compounds. In the ^13CNMR,there are no differences between the two kinds of compounds ,and the peaks of the two - CH groups don＇ t overlap. Conclusions： ^1HNMR technique is a sensitive, convenient, accurate, effective determined method for druggery structure.