RP-HPLC法测定玄参不同部位中2种皂苷的含量

RP-HPLC determination two triterpene glycosides in the different parts of Scrophularia ningpoensis Hemsl.

目的:建立玄参不同部位中2种皂苷 scrokoelzisideA 和 scrokoelziside B 的含量测定方法。方法:采用国产 YWG C_(18)色谱柱(250mm×4.6 mm,10μm),流动相为乙睛-水,梯度洗脱(0 min,乙睛-水比例为46:54;5 min,55:45),流速1 mL·min^(-1),双波长检测(0～10 min,251 nm;11～20 min,210 nm),柱温28℃。结果:Scrokoelziside A 和 scrokoelziside B 的线性范围分别为0.273～8.19μg和0.247～7.4μg,平均加样回收率(n=5)分别为95.6%和96.7%。根中不含有2种皂苷,地上部分 scrokoelziside A 含量明显高于 scrokoelziside B,叶中 scrokoelziside B 的含量大于花和茎,scrokoelziside A 在叶和茎中含量无大的差异,4月茎、叶中2种成分含量略高于其他月份。结论:本文建立的测定玄参不同部位中2种皂苷含量的高效液相色谱法,快速准确。

Objective：To determine scrokoelziside A and scrokoelziside B in Scrophularia ningpoensis, several samples from different parts were examined by HPLC using gradient elution. Methods：Four parts were dealt by HPLC with YWG - C18 analytical column （250mm×4.6mm,10μm）. The mobile phase consisted of acetonitrile -water, gradient elution [ 0 min, acetonitrile - water （46：54 ） ; 5 min, acetonitrile - water （ 55： 45 ）. The flow rate was 1 mL·min^-1. The UV detection was set at 251 nm（0-10 min）and 210 nm（ 11 -20 min）. Results：The linear ranges were 0.273 - 8.19μ（r=0.9995, n = 6） for scrokoelziside A and 0.247 - 7.41μg（r= 0.9999, n = 6 ） for scrokoelziside B, the average recoveries（ n = 5 ）were 95.6% and 96.7%, respectively. There were no scrokoelziside A and scrokoelziside B in roots of Scrophularia ningpoensis. The content of scrokoelziside A was higher than that of scrokoelziside B in the aerial part;The content of scrokoelziside B in leaves is the highest. There were no significant differences between the contents of scrokoelziside A in leaves and stems. The contents of two compounds in April stems and leaves were higher than other colleetef in offten time samples. Conclusion ： The method is rapid and accu- rate, and it is suitable for determination of two triterpene glycosides in Scrophularia ningpoensis.