HPLC法测定五子益肾酒中补骨脂素和异补骨脂素的含量

HPLC determination of psoralen and isopsoralen in Wuzi Yishen wine

目的:建立五子益肾酒中补骨脂素和异补骨脂素的高效液相色谱含量测定方法。方法:采用 Inertsil ODS-3C_(18)色谱柱(150 mm×4.60 mm,5μm),流动相为甲醇-水-磷酸(40:60:0.1);柱温35℃;流速:10 mL·min^(-1);检测波长为246 nm。结果:补骨脂素和异补骨脂素进样量均在0.047～0.233μg范围内线性关系良好,相关系数均为0.9999;平均回收率分别为98.44%和99.70%,RSD 分别为1.2%和2.0%。结论:该方法简便易行,重现性好,测定准确灵敏。

Objective：To establish the HPLC method for the determination of psoralen and isopsoralen in Wuzi Yishen wine, Method ：The Inertsil ODS -3 C18 column （150mm×4.60mm,5μm）was used. The mobile phase was methanol -water - phosphoric acid （40： 60： 0.1 ）and column temperature at 35 ℃, the flow rate was 1.0mL·min^-1 and the detection wavelength was 246 nm. Results ：The method had good linear relationship within the range of 0.047 to 0.233 μg for psoralen and isopsoralen with the correlation coefficients all of 0.9999. The average recov- eries were 98.44% and 99.70% , RSD was 1.2% and 2.0% respectively. Conclusion： The method is simple, ac- curate ,sensitive and has good repetition.