酸性染料比色法测定阿昔洛韦

Colorimetric determination of aciclovir with some acid dyes

目的:建立比色法测定阿昔洛韦浓度的方法,并用于实际药品、血浆及尿液中阿昔洛韦的测定。方法:在 NaH_2PO_4-Na_2HPP_4缓冲介质中,阿昔洛韦(ACV)与酚红(PR)、氯酚红(CPR)反应,形成离子缔合物,溶液颜色发生明显改变,最大吸收波长分别为558 nm(PR—ACV 体系)、574 nm(CPR—ACV 体系),在此波长处阿昔洛韦的浓度与增色程度呈良好线性关系。结果:在 PR—ACV、CPR—ACV 体系的最大吸收波长处,ACV 的浓度分别在0～2.86×10^(-5)mol·L^(-1)、0～2.37×10^(-5)mol·L^(-1)范围内遵守比尔定律,表观摩尔吸光系数分别为1.77×10~4L·mol^(-1)·cm^(-1)、1.64×10~4L·mol^(-1)·cm^(-1),检出限分别为8.21×10^(-7)mol·L^(-1)、4.50×10^(-7)mol·L^(-1)。结论:方法具有较高的灵敏度和良好的选择性,用于实际药品、血浆及尿液中阿昔洛韦的测定,结果满意。

Objective：To develop colorimetric method for determination of aciclovir and apply it to medicine samples and human serum and human urine. Methods：In NaH2PO4 -Na2HPO4 buffer medium, aciclovir（ACV） reacts with phenol red（PR） or chlorophenol red（CPR） to form an ion -association complex,the colours of solutions change obviously. The maximum absorption wavelength showed at 558 nm（ PR- ACV system）and of 574 nm（ CPR- ACV system）. The colours intensity adding is proportional to the concentration of aciclovir. Results：The concentration of aciclovir in the range of 0-2.86×10^-5 mol·L^-1 and 0-2.37×10^-5 mol·L^-1 obeyed Beerg law at the maxi-mum absorption wavelength of PR - ACV and CPR - ACV system. The apparent molar absorptivity were 1.77×10^4 L·mol^-1·cm^-1 and 1.64×10^4L·mol^-1·cm^-1,and the detection limit are 8.21×10^-7mol·L^-1 and 4.50×10^-7mol·L^-1 respectively. Conclusion ：The method showed high sensitivity and fairly good selectivity. The method is applied to the determination of aciclovir in medicine samples and human serum and human urine, with satisfactory results.