人工快速渗滤系统中硝化菌群脱氮作用解析

Analysis of nitrifying bacteria community denitrogenation action in constructed rapid infiltrition system

采用16SrDNA的变性梯度凝胶电泳（DGGE）技术研究人工快速渗滤系统（CRI）中硝化菌群（氨氧化菌和亚硝酸氧化菌）的空间分布规律．结果表明，采用机械破壁、酚氯仿抽提和巢式PCR的方法处理样品，为后续的DGGE提供了理想的模板；DGGE图谱显示，氨氧化菌种群随着滤层深度的增加先增多后减少，在20～60cm存在10种左右，多样性最丰富，是亚硝化作用的主要区域；亚硝酸氧化菌的多样性则相对贫瘠，主要分布在50cm及更深的范围，优势种群3-4种，是硝化作用的主要场所．

Adopting 16S rDNA of denaturing gradient gel electrophoresis （DGGE） technology to reveal nitrifying bacteria community （ammonium oxidizing bacteria and nitrite oxidizing bacteria） denitrogenation action in constructed rapid infiltrition system （CRI）. Adopting mini-beadbeater, phenol/chloroform extraction and nested PCR to treat the samples was ideal for DGGE. The microbial community of ammonium oxidizing bacteria increased and then decreased from top to bottom, 10 species existed in 20-60cm, where diversified property were most plentiful and was the main area for nitrosation. But diversified property of nitrite oxidizing bacteria was poor and 3-4 species were found below 50cm, where nitrification action existed.