斑点金标免疫渗滤新技术检测家畜血吸虫病的效果试验

Detection of circulating anti-schistosome antibodies in livestock using Dot Immunogold Filtration Assay

斑点金标免疫渗滤新技术（简称金标法）检测家畜血吸虫病抗体，在本省进行了特异性、敏感性、符合率等试验。金标法能检出人工感染犬血吸虫14d后的血吸虫病抗体，检出率为100％，可用于血吸虫病早期诊断。粪孵100头份血吸虫病阳性的牛血纸，用金标法检测全部为阳性，阳性符合率为100％；在非疫区的2个县分别采牛血纸100份，用金标法进行检测，未查出血吸虫病阳性牛，阴性符合率为100％。在金标法检测4000份牛血纸，检出阳性牛为129头，再对这129头牛采粪孵化，有血吸虫毛蚴的牛128头，金标法与病原学检查法阳性符合率为99．2％（99％～100％）。在本省54个血防疫区县推广应用，取得了显著的社会经济效益。

A new dot immunogold filtration assay（DIGFA） technology to detect circulating anti-schistosome antibodies in livestock was carried out for evaluation of its sensitivity, specificity, and consistency. It could detect the circulating anti-schistosome antibodies in blood paper lixivium from cattle 14 days post infection with schistosome cercaria while it could detect cerearia faeces hatching miracidium（FHM） for 42-56 days. DIGFA＇s detection rate is 100%, and suitable for the early diagnosis of animal＇s schistosomiasis. In 100 blood paper positive samples of cattle infected by schistosomiasis diagnosed with FHM method, the positive rate of DIGFA detection was 100%, which indicated that the coincidence rate is 100% between DIGFA and FHM methods. In the same study, out of 4 000 blood paper samples of cattle in epidemic areas of this disease 129 schistosomiasis positives as examined with DIGFA. The follow-up study showed that 128 infected cattle were positive as detected with FHM method, the consistent rates bewteen DIGFA and FHM is 99.2%. DIGFA method has been used in 54 counties of epidemic areas of infectious schistosomiasis, and yielded obvious socioeconomic benefits.