我国短季棉品种的RAPD指纹图谱分析

Analysis of RAPD Fingerprinting on Shortseasoned Cotton Cultivars in China

本文选用了18个随机引物,对25个我国主要的短季棉品种作了RAPD多态性分析。并对各品种的指纹图谱进行了聚类和相似性分析。结果表明,大部分短季棉品种与其系谱吻合。主要从来自美国的金字棉中选育而成,它再次反映了我国现在推广的短季棉品种遗传基础比较狭窄,亟待发掘新的早熟棉基因资源。研究结果可对杂种优势利用的亲本选配提供理论依据。

RAPD(Random Amplified Polymorphic DNA) technique was used to detect the genetic diversity of 25 short-seasoned cotton cultivars in China. The results indicated that 25 short-seasoned cotton cultivars could be divided into four subgroups based on cluster and similarity analysis of RAPD fingerprinting of each cultivar amplified with 18 random primers. Most of the tested cultivars were consistent with their pedigrees, i. e, most of short-seasoned cotton cultivars were developed from King cultivar introduced from USA. It is further testified that the genetic basis of popularized short-seasoned cotton cultivars in China is rather narrow and it is essen-sial to find some new germplasms for the development of new cultivars. The study is also valuable in the choice of parents for the development of cotton cultivars and hybrids.