流式细胞术检测特发性血小板减少性紫癜患者血小板相关免疫球蛋白及T淋巴细胞亚群

Determination of platelet-associated immunoglobulin and T lymphocyte subgroups in patients with idiopathic thrombocytopenic purpura by flow cytometry

目的:应用流式细胞术检测特发性血小板减少性紫癜(ITP)患者外周血中的血小板相关免疫球蛋白(PAIg)水平和T淋巴细胞亚群分布情况。方法:应用三色免疫荧光多参数流式细胞术检测ITP患者(n=30)和对照者(n=30)外周血PAIg水平和T淋巴细胞免疫表型(CD3+、CD3+CD4+及CD3+CD8+)。结果:ITP患者外周血PAIgG、PAIgM及PAIgA的平均荧光强度均显著高于正常对照组(P<0.01);与对照组比较,ITP患者外周血CD3+、CD3+CD4+亚群明显减低(P<0.05),CD3+CD8+亚群明显升高(P<0.05),而CD3+CD4+/CD3+CD8+比值显著减低(P<0.01)。结论:ITP患者PAIg水平显著增高,且存在T淋巴细胞亚群分布漂移。此方法简便易行,有助于ITP的诊断。

Objective：To determine the level of platelet-associated immunoglobulin （PAIg）and distribution of T lymphoeyte subgroups in patients with idiopathie thromboeytopenie purpura （ITP）by muhiparameter flow eytometey （FCM）with three-eolor immunofluroseenee.Methods：The levels of PAIg ineluding PAIgG.PAIgA and PAIgM and T lymphoeyte immunophenotype were respeetively determined in peripheral blood from patients with ITP （n=30） and healthy people as eontrol （n=30）.Results：Signifieant inerease was observed for level of mean fluoreseenee intensity of PAIgG,PAIgM and PAIgA in patients with ITP （P〈0.01）.In addition, eomparasions with the eontrols,the pereentage of CD3^＋,CD3^＋CD4^＋ deereased （P〈0.05）and the pereentage of CD3^＋CD8^＋ inereased （P〈0.05）in patients with ITP,whereas CD3^＋CD4^＋/CD3^＋CD8^＋ inereased signifieantly（P〈0.01）.Conclusion：There were signifieant elevation in level of PAIg and distribution exeursion in T lymphoeyte subgroups in patients with ITP.The method is simple and easy to perform,and helpful for the diagnosis of ITP.