一氧化氮在大鼠肝肺综合征发病机制中的作用研究

Role of nitric oxide in the pathogenesis of hepatopulmonary syndrome in rats

目的探讨一氧化氮(NO)在大鼠肝肺综合征(HPS)发病机制中的作用。方法应用放射免疫分析法检测HPS大鼠血浆和肝组织、肺组织匀浆中NO的水平。结果(1)HPS大鼠血浆和肝组织、肺组织匀浆中NO水平动态升高。(2)各阶段血浆和肝组织、肺组织匀浆中NO水平与谷丙转氨酶(ALT)、总胆红素(TB IL)呈正相关,出现腹水者血浆和肝组织、肺组织匀浆中NO水平高于未出现腹水者。结论在HPS形成过程中,血浆和肝组织、肺组织匀浆中NO水平持续升高,与肝功能受损状态和腹水形成有关,提示扩血管物质NO可能参与HPS的发生。

Objective To explore the role of nitric oxide （NO） in the pathogenesis of hepatopulmonary syndrome （HPS） in rats. Methods HPS model rats were induced by common bile duct ligation （CBDL）. Liver function and the pathological changes of liver and lung were observed. The concentrations of NO in plasma, liver tissues and lung tissues were detected by radioimmunoassay. Results （ 1 ） The levels of plasma, liver and lung NO were gradually increased from the third week to fifth week after CBDL. （2） Plasma,liver and lung NO were positively correlated with ALT and TBIL. The levels of NO in blood,liver and lung of rats with ascites were higher than that without ascites. Conclusions The levels of NO in plasma,liver and lung were increased during the process of HPS,which was correlated with impaired liver function and ascites. These results suggested that the combinative role of contractor NO was involved in the pathogenesis of HPS.