毛细管气相色谱法同时测定保健食品中的12种有机溶剂残留量

Simultaneous determination of 12 kinds of residual organic solvents in health food by capillary gas chromatography

目的：建立保健食品中的12种有机溶剂残留量同时测定的方法。方法：采用顶空进样毛细管柱气相色谱法，测定了保健食品中正己烷、环己烷、丙酮、四氢呋喃、乙酸乙酯、甲醇、异丙醇、乙醇、苯、甲苯、乙酸丁酯、正丁醇12种有机溶剂残留量，以N．N-二甲基甲酰胺为溶剂，色谱柱为CPWAX58CB毛细管柱（30m×0．32mm×0．25μm），检测器为F1D。结果：各有机溶剂色谱的分离度均大于1．5，线性关系良好（r〉0．99），平均回收率为95．1％～103．2％．RSD为1．4％～4．7％（n=6）。结论：本方法简便准确，灵敏度高，适用于保健食品中有机溶剂残留量的同时测定。

Objective：To establish a determination method for 12 kinds of residual organic solvents in health food simultaneously. Methods：The concentrations of 12 kinds of residual organic solvents including n - hexane, cyclohexane, acetone, tetra- hydrofuran, ethyl acetate, methanol, iso - propanol, thanol, benzene, toluene, butylacetate, n - butanol in health food were determined by headspacecapillary gas chromatography with CP WAX 58CB column （30 m ×0. 32 mm×0. 25 μm） and a solvent of N,N - dimethylformamide, a detector of FID. Results： These 12 kinds of residual organic solvents were separated effectively. There was a good linearity （r 〉0. 99）, the average recovery rate was in the range of 95.1% - 103.2%, the RSD of precision was less than 5%. Conclusion ：The method is simple, accurate and of high sensitivity. It is suitable for the determination of 12 kinds of residual organic solvents in health food simultaneously.