浙江省2006年柯萨奇B3病毒VP1基因分析

Analysis of VP1 gene of coxsackie virus B3 for aseptic meningitis prevailing isolated from Zhejiang Province in 2006

目的：研究2006年浙江省无菌性脑膜炎的病原柯萨奇B3（CoxB3）分离株（Zhejiang／52／06）的VP1基因特征。方法：采用RD、Hep-2细胞对患者脑脊液和粪便标本进行病毒分离，逆转录-聚合酶链反应（RT—PCR）扩增病毒VP1基因并进行序列测定，用BioEdit等软件分析处理。结果：浙江省柯萨奇B3分离株的VP1基因852个核苷酸，编码281个氨基酸，与北京、哈尔滨的CoxB3分离株核苷酸同源性在79．1％～79．2％，与欧美国家分离株的核苷酸同源性在78．5％～81，7％，与乌兹别克斯坦分离株核苷酸同源性为82．7％。进化树分析显示，浙江省柯萨奇B3分离株（Zhejiang／52／06）与B3／Uzbekistart／99、B3／Germany／PD00分离株亲缘关系最近。结论：柯萨奇B3分离株的变异特征及亲缘进化，分析对无菌性脑膜炎的流行病学研究具有重要意义。

Objective：To analyse the genetic characterization of the VP1 gene of Coxsackie B3 virus causing meningitis isola- ted from Zhejiang Province in 2006. Methods：The specimens of the cerebrospmal fluid（CSF） and faces were inoculated in Hep - 2 and RD cell lines and two strains of Coxsackie B3 virus were isolated. Viral RNA was extracted and the VP1 gene was amplified by RT- PCR. The sequence of the VP1 gene was determined and compared with BioEdit software. Results：The vplgene of Coxsackie virus B3 isolate from Zhejiang province was 852 bp, with a deduced product of 281 amino acids. Compared with the Beijing and Haerbin isolates of Coxsackie virus B3, the homology of nucleotide acids was between 79. 1% - 79. 2%. The homology was 78.5% - 81.7% and 82. 7% when compared with the isolates from the Occident and Uzbekistan, respectively. Phylogenetic analysis revealed that the evolutional relationship of the Coxsackie virus B3 isolated from Zhejiang Province was closest to the B3/Uzbekistan/99 and B3/Germany/PDO0 strains. Conclusion： The variation and phylogenetic analyses of the Coxsackie virus B3 are of great importance to the epidemiological study of aseptic meningitis.