磺胺甲恶唑和甲氧苄啶在水产品中残留的检测

Determination of residues of sulfamethoxazole and trimethoprim in water product

目的：建立同时检测磺胺甲恶唑和甲氧苄啶的液相色谱串联质谱（LC-MS／MS）方法。方法：样品经乙腈和三氯甲烷溶剂提取后进行液相色谱质谱分析。液相色谱柱为CN柱，甲醇为流动相，流速300灿。rain；质谱采用选择反应监测技术，磺胺甲恶唑采用负离子模式，监测的离子对为m／z252〉m／z157；甲氧苄啶采用正离子模式，监测的离子对为，m/z291〉m/z230，m／z258。结果：磺胺甲恶唑的检出限为0．7μg／L，线性范围为7～770μg/L，回收率为89．1％；甲氧苄啶的检出限为0．8μg/L，线性范围为0．8～800μg／L，回收率为92．0％。结论：利用液相色谱串联质谱技术测定鱼肉中的磺胺甲恶唑和甲氧苄啶残留的方法具有高选择性、高灵敏度、快速等特点，具有实用性。

Objective：A method for simultaneous determination of sulfamethoxazole and trimethoprim by liquid chromatography coupling tandem mass spectrometer was developed. Methods：Sample extracted by aeetonitrile and chloroform was analyzed by LC - MS/MS. Column was CN, mobile phase was methanol, flow rate was 300 i.d/min. Selective reaction monitoring technique （SRM） was adopted. Negative scan mode was used for sulfamethoxazole （SMZ）, and quantitative ion pair was m/z252 〉 m/z157 ; positive scan mode was used for trimethoprim （TMP）, and quantitative ion pairs were m/z291 〉 m/z230 and m/z291 〉 m/ z258. Results：For sulfamethoxazole and trimethoprim, detection limit was 0. 8 μg/L and 0. 7 μg/L, respectively. Linear range was 7 -770μg/L and 0. 8 -800 μg/L, respectively and recovery was 89. 1% and 92.0%, respectively. Condusion：This is a highly selective, highly sensitive, and rapid detecting method for sulfamethoxazole and trimethoprim. It can be applied to detect residues of sulfamethoxazole and trimethoprim in fish.