气相色谱法测定韭菜中五氯硝基苯残留研究

Determination of pentachloronitrobenzene in leek by gas chromatography

目的：建立韭菜中五氯硝基苯的气相色谱分析方法。方法：韭菜样品用丙酮／正己烷（1＋1，v／v）均质提取，用浓硫酸净化，采用DB-1701毛细管柱分离，电子捕获检测器检测。结果：本方法测定韭菜中五氯硝基苯，在5～150ng／ml浓度范围内，线性回归方程Y=268．4X-53．2，相关系数r=0．9994，线性回归良好；回收率87．6％～92．2％，相对标准偏差小于4．9％，该方法的检出限为0．5μg／kg。结论：本方法简便、快速、灵敏，同时具有良好的回收率和重现性。

Objective：To establish a simple, accurate and sensitive method for the determination of pentachloronitrobenzene residue in leek by gas chromatography. Methods： Pentachloronitrobenzene residue was extracted with acetone/hexane （ 1 ＋ 1, v/ v） and partitioned with hexane. The extract was cleaned up with H2SO4, separated on a DB - 1701 column, detected by a micro electron capture detection. Results：The linear range was 5.0 ～ 150.0 ng/ml and the limit of quantification was 0.50 μg/kg for pentachloronitrobenzene. The extraction recoveries were between 87. 6%～ 92. 2% and the RSD was less than 4. 9%. Conclusion： This method is simple, rapid and sensitive with satisfactory recoveries and reproducibility.