广西细胞色素P4501A1、谷胱甘肽转硫酶基因多态性和急性髓系白血病的相关性研究

Association between the polymorphisms of cytochrome P4501A1 and glutathione S-transferase M1,T1 Genes and acute myeloid leukemia in Guangxi

目的探讨广西地区生物代谢酶细胞色素P4501A1、谷胱甘肽转硫酶M1、T1基因多态性和急性髓系白血病的相关性。方法采用病例对照研究方法,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对98例急性髓系白血病（AML）患者以及120例健康对照者的CYPlAl Msp1多态（T264C）、GSTMI和GSTT1等基因的多态分布进行分析。结果AML组的CYP1A1基因（T264C）位点等位变异的频率及GSTTI缺失基因型的频率与对照组差异无统计学意义（P〉0.05）,而GSTMI缺失基因型频率与对照组比较差异有统计学意义（P〈0.05）。携带CYP1A1（T264C）位点突变基因型与GSTMI、Tl缺失基因型的个体患AML的风险增加（OR=1.981,95%CI：1.045~3.758）。结论单一的CYPIAl Msp1多态等位变异基因型或GSTT1缺失基因型与AML易感性可能不相关;GSTMI缺失基因型与AML易感性可能相关;携带CYP1A1突变基因型与GSTMI、Tl缺失基因型可能是ANLL发病的易感因素之一。

Objective To study the association between metabolic enzymes polymorphisms of cytochrome P4501A1 and glutathione S-transferase M1 ,Tlgenotypes and susceptibility to acute myeloid leukemia in Guangxi. Methods Using case-control methodology,we studied 120 healthy controls and 98 patients with acute myeloid leukemia. The frequencies of the genotypes were detected by PCR or PCR-RFLP techniques. Results The differences of the variation frequencies of CYP1A1 gene （MspI polymorphisms ） and GSTI＇I between patients and healthy controls were not statistically significant （P 〉 0.05 ）. However,the null genotype of GSTM1 in AML group was significantly different from the controls （P 〈 0. 05 ）. （T264C） varlation of CYP1 A1 gene combined with the null genotype of GSTM1, T1 increased the risk of AML （OR value 1.981,95% CI ： 1. 045 - 3.758）. Conduslon These results indicated that both the variation of CYP1A1 gene or the null genotype of GSTYI alone might not be associated with the susceptibility of AML. The null genotype of GSTM1 might be associated with the susceptibility of AML. Variation of CYP1A1 gene combined with the null genotype of GSTM1 ,T1 might serve as a risk factor to the etiology of AML.