男性不育患者生精细胞巨细胞病毒感染检测分析

To detection the infection of human cytomegalovirus in spermatogenic cells in the semen of infertile male

目的:探讨男性不育患者精液中生精细胞人类巨细胞病毒(HCMV)感染状况。方法:收集99例精液检查中发现较多生精细胞男性不育病例精液,洗涤收集细胞后提取DNA,用聚合酶链反应(PCR)行HCMV筛查,阳性者精液细胞涂片用免疫细胞化学(ICC)做HCMV抗原标记。结果:99例精液样本PCR检测HCMV阳性10例。10例HCMV阳性病例精液细胞涂片ICC标记生精细胞HCMV晚期抗原均见阳性表达,HCMV早期抗原均为阴性表达。HCMV阳性病例精液均发现较多未成熟生精细胞,并有不同程度的病理性损害。精子密度HCMV阳性组显著低于阴性组(P<0.05)。HCMV阳性患者血清HCMV-IgG均阳性,HCMV-IgM均阴性。结论:生精细胞HCMV感染时可出现不同程度的病理性损害,影响生精功能,可能是引起男性不育的原因之一。

Objectives： To detect the infection of human cytomegalovirus （HCMV） of spermatogenic cells in the semen of infertile males. Methods： 99 semen samples of infertile males were collected and more spermatogenic cells were found through semen routine assaying. Then the cells were washed and concentrated to extract DNA. HCMV DNA were detected by polymerase chain reaction（PCR）. Immunocytochemistry（ICC） technique was used to detect the expressions of HCMV antigen in positive infected semen cells. Results ： PCR test revealed 10 HCMV positive cases in 99 semen samples. Among all 10 HCMV PCR positive cases, HCMV late antigen has positive expression in spermatogenic cells by ICC assaying whereas HCMV early antigen has negative expression. Among all HCMV positive cases, many immature spermatogenic cells were found in semen samples and have some pathologic variation. Sperm density of HCMV positive group was significantly lower than that of HCMV negative group（P 〈 0. 05 ）. Among all HCMV positive cases, HCMV - IgG was positive and HCMV - IgM was negative in blood. Conclusion： Spermatogenic cells with HCMV infection may cause pathologic lesions, and affect spermatogenesis. And these lesions may contribute to male infertility.