摘要
目的研究维拉帕米对人胃癌耐药细胞系SGC7901/VCR多药耐药性的逆转效应。方法利用RT-PCR、免疫组化以及MTT等方法,观察维拉帕米对体外培养SGC7901/VCR细胞系耐药性的逆转作用。结果RT-PCR、免疫组化和MTT结果表明维拉帕米可以使SGC7901/VCR的MDR1基因表达水平和P-gp糖蛋白表达降低,细胞对丝裂霉素、替加氟等药物的敏感性明显增加。结论维拉帕米在体外有逆转SGC7901/VCR细胞多药耐药的效应。
Objective To explore the reversal effect of verapamil on muhidrug resistance （MDR） in drug-resistant human gastric cancer cell line SGC7901/VCR and its mechanisms. Methods Expression of multidrug resistance 1 （MDR1） gene was detected using reversal transcription polymerase chain reaction（RT-PCR）. Expression of P-glycoprotein（P-gp） was detected by immunohistochemistry method. Tetrazolium blue （MTT） assay was used to evaluate the sensitivity of cells to anticancer agents, mitomycin and tegafur. Results Expression of MDR1 gene and P-gp were knockdown by verapamil. MTT showed that the sensitivity to anticancer agents were promoted by verapamil. Conclusion Verapamil has potent reversal effect on MDR in SGC7901/VCR via inhibiting the function of MDR1 and P-gp, and enhancing the sensitivity to anticancer agents, mitomycin and tegafur.
出处
《国际消化病杂志》
CAS
2008年第2期166-168,共3页
International Journal of Digestive Diseases
关键词
多药耐药
P-gP糖蛋白
逆转
Multidrug resistance（MDR）
P-glycoprptein（P-gp）
Reverse
