接种固氮菌对甘蔗组培苗氮营养生理特性的影响

Effects of Inoculating Azotobacter on the Physiological Characteristics of Nitrogen Nutrition in the Tissue Cultured Seedlings of Sugarcane

[目的]研究接种固氮菌对甘蔗组培苗氮营养生理特性的影响。[方法]将2种内生固氮菌B16L与B8R接种到甘蔗栽培品种G16、R16与R22的组培苗中,研究这些组培苗的氮营养生理特性的变化。[结果]于分蘖期和拔节期的测定结果表明,R16与R22+1叶的叶绿素含量、Ca2+-ATP酶活性处理均大于对照,G16+1叶的Ca2+-ATP酶活性处理低于对照。相对于分蘖期,拔节期不同品种的处理与对照的+1叶的叶绿素含量、Ca2+-ATP酶活性都有所升高。不同品种的处理与对照+1叶的硝态氮含量没有规律变化。[结论]接种内生固氮菌改善了R16与R22健康组培苗的一些氮营养生理特性,但对G16的影响不大。

[Objective] The aim was to study the effects of azotobacter inoculation on the physiological characteristics of nitrogen nutrition in the tissue cultured seedlings of sugarcane. [Method] Two species of endophytic azotobacters B16L and BSR were inoculated into to the tissue cultured seedlings of sugarcane euhivars G16, R16 and R22 so as to study the changes of physiological characteristics of nitrogen nutrition in these tissue cultured seedlings. [Result] Determinations in tillering and jointing stages showed that the chlorophyll content and Ca^2＋-ATPase activity in the ＋ 1 leaves of R16 and R22 in treatments were bigger than that in CK and the Ca^2＋ -ATPase activity in the ＋ 1 leaf of G16 in treatment was lower than that in CK. Compared with tillering stage, the chlorophyll content and Ca^2＋ -ATPase activity in the ＋ 1 leaves of different euhivars in treatments and CK in jointing stage had some increase. The nitrate nitrogen contents in the ＋ 1 leaves of different eultivars in treatments and CK changed irregularly. [Conelusionl The inoculation ofen- dophytie azotobaeter improved some physiological characteristics of nitrogen nutrition in the healthy tissue cultured seedlings of R16 and R22, but had little effect on G16.