阿托伐他汀对大鼠脑缺血后补体水平的影响

Effects of atorvastatin on complement in rats after the cerebral ischemia infarction

目的 研究大鼠脑缺血后补体表达的变化规律，探讨阿托伐他汀对大鼠脑缺血后补体表达的影响。方法 160只健康成年S-D大鼠随机分为正常组、假手术组、缺血组、治疗组，缺血组和治疗组分别再分为缺血后6h、12h、24h、48h、3d、5d、2同七个小组。线栓法制备大鼠大脑中动脉闭塞（The middle cerebral artery occlusion，MCAO）模型，缺血2h后退出线栓，假手术组不插入线栓。麻醉清醒后大鼠出现对侧肢体瘫痪表明造模成功。治疗组在造模成功后开始口饲阿托伐他汀钙10mg／d，每日1次，连用2周。缺血组不给予阿托线他汀类药物。对缺血组和治疗组大鼠不同时点进行神经功能缺失评分，然后断头取脑，免疫组化法检测脑内补体C1q和C3d的表达情况。结果正常组大鼠脑内有少量补体表达，假手术组补体表达与正常组比较无统计学意义（P〈0．05），脑缺血后脑内补体C1q和C3d的表达水平逐渐增加，至缺血后24h达高峰，5d左右恢复至正常水平。缺血组补体C1q、C3d的表达明显高于假手术组（P〈0．05），治疗组补体C1q和C3d的表达明显低于缺血组（P〈0．05），治疗组神经功能评分明显低于缺血组（P〈0．05）；结论脑缺血后脑内补体C1q、C3d的表达逐渐升高，阿托伐他汀可以抑制脑内补体的激活，改善神经功能。

Objective To study the characteristics of complement expression and the effects of atorvastatin on the complement in rats after the cerebral ischemic infarction. Method One hundred and sixty adult healthy Sprague-Daweley rats were randomly divided into normal group, sham-operated group, ischemia group and treat group. The ischemia group and treatment group were sub-devided into 6 h, 12 h, 24 h, 48 h, 3 d, 5 d, 2 week, respectively. The middle cerebral artery occlusion （MACO） model was induced by using filament method, and the thread was withdraw from the middle cerebral artery after occlusion 2 h. Rats in the sham-operated groupdid not have thread inserted. After wakefulness, the animals The successful of animal model produced was evidenced by the paralysis of contralateral limbs. The animals model in the treatment group treated with Atorvastatins 10 mg once a day for 2 weeks and, rats in the ischemia group did not receive Atorvastatin. The neurological deficit scoring was measured at different intervals in the ischemia and treat ment groups. The expressions of complement Clq and C3 d protein in the brain of the rats in 4 groups were measured by immunohistochemical methods. Result There were a few complement expression in the brain of normal rats, showing no significant difference observed between sham-operated group and normal group （ P 〈 0.05）. The complements （ C1 q and C3 d） expressied increasubgkt after cerebral ischemia injury, peaked 24 h after ischemia, and returned to normal levels 5 d after ischemia. The expression of complement C1 q and C3 d in the ischemia group were significantly higher than those in sham-operated group （ P 〈 0.05）, The expression of complement C1 q and C3 d in treat ment group were signnificantly lower than those in the ischemia group （ P 〈 0.05）. The neurological deficit score in treatment group were sigrmificantly lower than that of ischemia group. Conclusions The expressions of complement C1 q and C3 d were increase gradually after cerebral ischemia in the rats, Atorvastatin can inhibit the complement activation , improve neuro logical function of rats.