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不同脂肪酸饮食导致大鼠肝脏脂代谢紊乱的实验研究 被引量:4

Lipid metabolic disorder in the livers of rats induced by different fatty acid diets
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摘要 目的探讨不同类型高脂肪酸饮食对大鼠肝脏过氧化物酶体脂肪酸β-氧化的影响。方法用高不饱和和高饱和脂肪酸饮食饲养大鼠24w,观察体重(BW),血清总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA),肝脏过氧化氢酶、脂酰CoA氧化酶和过氧化物酶体脂肪酸β-氧化活性的变化及肝脏超微结构的改变。结果各实验组血清BW、TG、TC、FFA较对照组有不同程度升高(P<0.01)。各实验组大鼠肝脏过氧化氢酶、过氧化物酶体脂肪酸β-氧化、脂酰CoA氧化酶活性较对照组明显升高(P<0.05,P<0.01)。24w后高脂饮食组大鼠肝细胞内有数量不等的脂滴储积。结论高不饱和脂肪酸、高饱和脂肪酸饮食长期摄入可导致大鼠脂质代谢紊乱,肝脏中的过氧化氢酶、脂酰CoA氧化酶和过氧化物酶体脂肪酸β-氧化的活性升高。 Objective To explore the effect of high different type of fatty acid diet on the activity of peroxisome fatty acid β-oxidation in the livers of rats. Methods High saturated fatty acid and high unsatured fatty acid diets had been administratered to different group of rats for 24 weeks respectively. The alterations of body weight (BW) , total cholesterol ( TC ) , triglyceride ( TG), and free fatty acid ( FFA ) in serum and the alterations of the activities of peroxisome fatty acid β-oxidation,catalase and acyl-CoA oxidase in the livers were observed. Results BW,TC,TG, FFA in experimental groups were higher than those in control group in the same period. The activities of peroxisome fatty acid β-oxidation ,catalase and acyl-CoA oxidase in livers of the rats in experimental groups were increased more significantly than those in control group on 24 week. Conclusions Long period high saturated fatty acid and high unsatured fatty acid diets can result in metabolic disorder of lipid and the increasing of activities of peroxisome fatty acid β-oxidation,catalase and acyl-CoA oxidase of liver.
出处 《中国老年学杂志》 CAS CSCD 北大核心 2008年第8期742-744,共3页 Chinese Journal of Gerontology
基金 河北省自然基金资助项目(编号303552)
关键词 不饱和脂肪酸 饱和脂肪酸 过氧化物酶体 脂肪酸β-氧化 Unsaturated fatty acid Saturatod fatty acid Catalase Peroxisomal fatty acid β-oxidation
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参考文献10

  • 1李静,宋光耀,姜玲玲,张文杰,冯志山.实验性糖尿病大鼠肝过氧化物酶体脂肪酸β-氧化及D双功能蛋白活性变化的初步研究[J].生物化学与生物物理进展,2004,31(11):1024-1030. 被引量:8
  • 2Kunau WH,Dommes V,Schuiz H.Beta-oxidation of fatty acids in mitochondria,peroxisomes,and bacteria: a century of continued progress[J].Prog Lipid Res,1995;34:267-342.
  • 3关紫烽,姜波,白卉.食用动物油与植物油中脂肪酸组成的比较研究[J].中国公共卫生,2002,18(12):1438-1438. 被引量:10
  • 4Storlien LH,Higgins JA,Thomas TC,et al.Diet composition and insulin action in animal models[J].Br J Nutr,2000;83(Suppl1):S85-90.
  • 5Jump DB,Clarke SD.Regulation of gene expression by dietary fat[J].Annu Rev Nutr,1999;19:63-90.
  • 6Ishii H,Horie S,Suga T.Physiological role of peroxisomal beta-oxidation in liver of fasted rats[J].J Biochem,1980;87:1855-8.
  • 7Debeet LJ,Mannaetts GF.The mitochondrial and peroxisomal pathway of fatty acid oxidation in rat liver[J].Diabete Metabol,1983;9:134-40.
  • 8Ouali F,Djouadi F,Merlet-Benichou C,et al.Regulation of fatty acid transport protein and mitochondrial and peroxisomal beta-oxidation gene expression by fatty acids in developing rats[J].Pediatr Res,2000;48(5):691-6.
  • 9Schulz Y,Flatt JP,Jèquier E.Failure of dietary fat intake to promote fat oxidation: a factor favoring the development of obesity[J].Am J Clin Nutr,1989;50:307-14.
  • 10Akbiyik F,Cinar K,Demirpence E,et al.Ligand-induced expression of peroxisome proliferator-activated receptor alpha and activation of fatty acid oxidation enzymes in fatty liver[J].Eur J Clin Invest,2004;34(6):429-35.

二级参考文献26

  • 1Jiang L L, Miyazawa S, Hashimoto T. Purfication and properties of rat D-3-hydroxyacyl-CoA dehydratase: D-3-hydroxyacyl-CoA dehydratase/ D-3-hydroxyacyl-CoA dehydrogenase bifunctional Protein. J Biochem (Tokyo), 1996, 120 (3): 633 ~641
  • 2Jiang L L, Kobayashi A, Matsuura H, et al. Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: mediumchain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase. J Biochem, 1996, 120 (3): 624 ~ 632
  • 3Jiang L L, Kurosawa T, Sato M, et al. Physiological role of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein. J Biochem (Tokyo), 1997, 121 (3): 506 -513
  • 4Yokota S, Asayama K. Proliferation of myocardial peroxisomes in experimental rat diabetes: a biochemical and immunocytochemical study. Virchows Archiv B Cell Pathol, 1992, 63 (1): 43 -49
  • 5Osmundsen H, Brodal B, Hovik R. A luminometric assay for peroxisomal beta-oxidation. Effects of fasting and streptozotocindiabetes on peroxisomal beta-oxidation. Biochem J, 1989, 260(1): 215 -220
  • 6Aebi H. Catalase. In: Bergmeyer H U ed. Methods of Enzymatic Analysis. New York: Academic Press, 1974. 673
  • 7Lazarow P B, De Duve C. A fatty acyl-CoA oxidizing system in rat liver poroxisomes; enhancement by clofibrate, a hypolipidemic drug. Proc Natl Acad Sci USA, 1976, 73 (6): 2043 -2046
  • 8Walusimbi-Kisitu M, Harrison E H. Fluorometric assay for rat liver peroxisomal fatty acyl-coenzyme A oxidase activity. J Lipid Research, 1983, 24 (8): 1077 -1084
  • 9Kunau W H, Dommes V, Sehuiz H. β-oxidation of fatty acids in mitochondria, peroxisomes, and bacteria: A century of continued progress. Prog Lipid Res, 1995, 34 (4): 267 -342
  • 10Singh I. Biochemistry of peroxisomes in health and disease. Mol Vell Biochem, 1997, 167:1 -29

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