全反式维甲酸联合丁酸甘油酯诱导滤泡状甲状腺癌的实验研究

Combination of all-trans retinoic acid with tributyrin induced human follicular thyroid carcinoma

目的探讨全反式维甲酸（ATRA）联合丁酸甘油酯（TB）诱导滤泡状甲状腺癌细胞株FTC-133钠／碘同向转运体（NIS）和甲状腺球蛋白（Tg）表达及其碘的摄取。方法单独使用1μmol／L ATRA或0．1，0．25，0．5，1mmol／LTB和联合使用该2种药物诱导FTC-13348h后，用实时定量PCR反应检测NISmRNA和唯mRNA的表达，蛋白印迹法检测NIS蛋白表达，放射免疫法检测Tg蛋白含量以及检测FTC-133诱导后摄碘变化。结果TB可诱导FTC-133NIS、Tg蛋白及mRNA的表达增高，并呈剂量依赖性。联合1μmol／LArllRA及各浓度，TB后较单独使用1μmol／LA耵认及，TB明显提高了FTC-133NIS、Tg蛋白及mRNA的表达，并且增加了FTC-133对^125Ⅰ的摄取。结论ATRA联合，TB有效增强了FTC-133的摄碘能力，为低分化甲状腺癌的放射性碘治疗提供了一条新的研究途径。

Objective This study was to evaluate the expressions of thyroid specific sodium/iodide symporter （NIS）, thyroglobulin （Tg） and radioiodide accumulation, induced by all-trans retinoic acid （ATRA） and （or） tributyrin （TB） in thyroid carcinoma cell line FTC-133. Methods Thyroid carcinoma ceil line FTC-133 was cultured in the presence of 1μmol/L ATRA and （or） 0.1,0.25, 0.5, 1 mmol/L TB respectively. After 48 h, the expressions of NIS mRNA and Tg mRNA were measured by Realtime RT- PCR, the protein of NIS was identified by Western blot and the protein of Tg was quantified by radioimmunoassay. Moreover, the radioactivity in FTC-133 thyroid carcinoma ceil line was measured. Results In FTC-133 thyroid carcinoma cells, TB alone could induce dependent expression of NIS mRNA, Tg mRNA and the proteins levels. When compared with either 1 μmol/L ATRA or TB alone, the combinations of 1 μmol/L ATRA and TB had higher expressions of the thyroid-specific genes mRNA and proteins and the accumulation of ^125I. Conclusions The combination of ATRA with TB might have the highest accumulation of radioiodide in FTC-133 thyroid carcinoma cell line. This method might possibly help in enhancing the radioiodine accumulation in poorly differentiated thyroid carcinoma cell.