复合振动预防去势大鼠骨质疏松的实验研究

Effect of compound vibration on ovariectomized sprague-dawley rats:a random controlled osteoporosis prevention study

目的:观察低强度复合振动对去势SD雌性大鼠生长期骨密度的影响。方法:4月龄SD雌性未育大鼠48只随机分为正常对照组(假手术组)、去势对照组以及4个去势振动组,每组8只。两对照组不作振动干预,4个振动组分别接振不同参数振动,干预时间13周。比较大鼠体重,腰椎、股骨骨密度,骨转换标志物(血清骨钙素、碱性磷酸酶、非前列腺酸性磷酸酶、Ⅰ型胶原C端肽)值。结果:实验开始后除正常对照组外,其余各组体重均明显增加。各组股骨骨密度均增加,差异无显著性意义,振动3、4组与正常对照组较去势对照组腰椎骨密度显著性增加。骨转换标志物各组差异无显著性意义。结论:一定的复合振动可以增加去势SD大鼠腰椎骨密度,具有潜在的预防骨质疏松作用。

Objective：To observe the effect of compound vibration on bone mineral density （BMD） of ovariectomized rats. Method： Fourty eight SD rats of 4-months old were randomly divided into 6 groups （n=8）： normal control group （NC, sham-operation group）, ovariectomized control group （OC）, and four ovariectomized plus vibration groups （OV）： group 1 （OV1,35Hz-45Hz, 0.05g-0.1g）, group 2 （OV2,35Hz-45Hz, 0.12g-0.21g）, group 3 （OV3,45Hz-55Hz, 0.05g-0.1g） and group 4（OV4,45Hz-55Hz, 0.12g-0.21g）. The experiment time was 13 weeks. The weight, BMD and serum bone-turn markers were compared among groups respectively.Result：From the 1st month, weight of all ovariectomized groups were greater than NC group（P=-0.000）. Femur BMD of each group was increased but with no statistical difference among groups. Only OV3 （P=0.005）, and OV4（P=0.001） improved the lumbar BMD compared with OC group, and reached the level of NC. Bone-turn marker failed to show a significant difference among all the groups.Conclusion：The compound vibration with the parameter of 45Hz-55Hz, 0.05g-0.1g and 0.12g-0.21g could maintain or improve lumbar BMD of ovariectomized growing rats. The vibration treatment may be used in prevention of osteoporosis.