胶质源性神经营养因子体外诱导小鼠胚胎中脑神经干细胞分化的研究

Experimental study on differentiation of mesencephalic neural stem cells induced by GDNF in vitro

目的:探索胶质源性神经营养因子(GDNF)在体外诱导小鼠胚胎中脑神经干细胞(M-NSCs)分化成多巴胺能神经元的培养方法,为M-NSCs移植治疗帕金森病(PD)提供实验依据。方法:在有血清条件下体外培养鼠胚M-NSCs,予以GDNF作诱导分化。TH免疫细胞化学鉴定,流式细胞术检测TH-ir阳性神经元的百分率。结果:M-NSCs向多巴胺能神经元分化的阳性率经流式细胞术检测结果,两组TH-ir阳性细胞比率A组(实验组,6只)为13.53%±1.53%;B组(对照组,6只)3.46%±0.77%,两组比较差异有显著性意义(P<0.01)。结论:GDNF可促进M-NSCs分化成多巴胺能神经元。

Objective：To explore the culture methods for the differentiation of mesencephalic neural stern cells （MNSCs） from embryonic mice into dopaminergic （DAergic）neurons in vitro,and to provide the experimental information for the treatment of Parkinson＇s disease by cell transplantation.nethod：M-NSCs were cultured and proliferated,then GDNF were used to induce differentiation in the condition of fetal bovine serum （FBS）.Immunocytochemical techniques were used to detect the TH-ir cells from NSC differentiation,and FCM to detect the percentage of TH- ir in all the cells. Result：The proportions of M-NSCs differentiated into DAergic neurons were 13.53%±1.53% in GDNF group, and 3.46%±0.77% in control group （P〈0.01）. Conclusion： GDNF can significantly increase the differentiation of DAergic neurons.