‘寒富’苹果的自花结实性及其S基因型

The Self-fruitfulness of‘Hanfu’ Apple and Identification of the S-Genotypes

以苹果‘寒富’品种及其亲本为试材,研究其自交亲和性并鉴定其S基因型。田间授粉试验结果显示,‘寒富’品种表现为自花结实,其母本‘东光’品种亦自花结实;而父本‘富士’品种自花授粉结实率极低,初步推断‘寒富’品种的自花结实性可能遗传自其母本‘东光’。利用PCR技术鉴定‘寒富’品种S基因型为S1S9。由于‘东光’品种存在同名异物问题,本研究除日本‘东光’品种外,对辽宁省果树研究所、吉林省果树研究所、沈阳农业大学等单位保存的中国‘东光’品种的S基因型进行了鉴定,发现除辽宁省果树研究所保存的‘东光’品种S基因型为S2S19,与‘寒富’品种无亲缘关系外,其它两个单位保存的‘东光’品种中均存在S9基因型,可能为‘寒富’杂交育种时的原始母本。

‘ Hanfu＇ apple cultivar （Malus x domestica） and its parents were used in this research to study its self-compatibility and identify their S-genotypes. The pollination test in the field showed that the ‘ Hanfu＇ is self-compatible, and its female parent ＇ Dongguang＇ is also self-compatible, but its male parent ＇ Fuji＇ is self-sterile as known. The S-genotype of ＇ Hanfu＇ was identified as S1 S9 by the PCR-based method. Also, the S-genotypes of all ＇ Dongguang＇ which are homonym were identified except the ＇ Toko＇ which came from Japanese and was named as ＇ Dongguang＇ in Chinese. The results showed the S-genotype of ＇ Dongguang＇ from Liaoning Research Institute of Pomology is S2S19, showing no relationship with ＇ Hanfu＇ , and the S-genotypes of ＇ Dongguang＇ cuhivars from Shenyang Agricultural University, and Jilin Research Institute of Pomology contain S9 allele, suggesting they are probably the female parent of‘ Hanfu＇.