摘要
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.
AIM： To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus （NGVEV） and the relationship between NGVEV and host cells. METHODS： The apoptosis of duck embryo fibroblasts （DEF） induced by NGVEV was investigated by fluorescence-activated cell sorter （FACS） and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide （PI）. RESULTS： By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells （Annexin V-FITC negative/PI negative）, early apoptotic cells （Annexin V-FITC positive/PI negative）, late apoptotic/necrotic cells （Annexin V-FITC positive/ PI positive） and dead cells （Annexin V-FITC negative/PI positive） through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.
基金
The National Natural Science Foundation of China, No. 39970561
The Key Projects in the National Science and Technology Pillar Program, 2007Z06-017
Program for New Century Excellent Talents from Universities, Chinese Ministry of Education, No. NCET-04-0906/NCET-06-0818
Fund of the Discipline Leaders of Sichuan Province, No. SZD0418
Culture Fund for Excellent Doctoral Dissertations of Sichuan Agricultural University, 2008scybpy-1
关键词
成纤维细胞
病毒性肠炎
细胞凋亡
治疗方法
Gosling viral enteritis
New type
Virus
Duck embryo fibroblasts
Apoptosis
Fluorescein annexin V-FITC/PI
