转基因何首乌毛状根对8种活性成分的生物转化研究

Biotransformation of Eight Active Constituents by Hairy Roots of Polygonum multiflorum Thunb

目的:利用转基因何首乌毛状根悬浮培养体系对青蒿素等8种活性化合物进行生物转化研究。方法:分别向预培养9d的何首乌毛状根培养体系中加入底物,共培养7d后终止转化,通过TLC和/或HPLC检测转化产物,使用柱色谱法分离纯化产物。结果:化合物呋喃橐吾酮(6)发生了生物转化,并分离得到两个转化产物;对苯二酚(7)被转化,分离并鉴定出1个转化产物;青蒿素(8)发生了转化,分离得到两个转化产物。结论:转基因何首乌毛状根悬浮体系中存在多种生物酶系统,可对外源底物进行糖基化、氧化、还原和羟基化修饰。

Objective： To investigate the biotransformation of eight active constituents chemicals by hairy roots of Polygonum multiflorum Thunb. Methods： Active compounds were added to the culture system of hairy roots of P. multiflorum after precuhured for 9 d, then they were co-cultured for another 7 d. The products were isolated and purified by column chromatography. Results ： Three of the eight active constituents were identified to be biotransformed, which were furannoligularenone （ 6）, 1,4 -benzenediol （7） and artemisinin （ 8 ）. HPLC analysis of the biotransformation of 6 showed two new peaks except the peak of substrate, then the corresponding two compounds were obtained; Substrate 7 was converted into arbutin. In the case of 8, there were two new peaks and the corresponding two monomers have been also isolated. Conclusion： The result showed that there were several enzymes in the culture system of P. multiflorum hairy roots, which could processed glycosylation, oxidation, reduction and hydroxylation reactions.