幽门螺杆菌培养及抗原蛋白的分离纯化

Culture of Helicobacter pylori and purification of antigen protein

幽门螺杆菌(Hpylori)是一种生存于人胃中的弯曲状菌,能导致人的慢性胃炎,消化性溃疡和胃癌,其治疗多用抗生素治疗,但其副作用较大.给奶牛免疫Hpylori疫苗后,其分泌的牛奶中含抗Hpylori的免疫球蛋白,能够预防和治疗人类Hpylori感染,且没副作用.在生产免疫乳时,Hpylori疫苗制备是基础性工作,并且在亚单位疫苗中,抗原蛋白的制备和分离纯化又最为关键.本文就Hpylori的培养(固体和液体培养)和抗原蛋白(尿素酶、空泡毒素、毒素相关蛋白和脂多糖)的理化性质、重组表达以及分离纯化方法进行比较研究.

Helicobacter pylori （H pylori） live in the stomach of human. It is a kind of curve bacteria which can lead to chronic gastritis, peptic ulcer and gastric cancer. Usually, antibiotics are used to treat the patients, but they have a lot of side effects, Milk of milch cow immunized with H pylori vaccine can prevent and treat the infection of H pylori without side effects, During the production of immune milk, the basic work is to prepare vaccine, During the preparation of sub-unit vaccine, key is to isolate and purify the antigen protein. In this article, we discuss the culture of H pylori （solid and liquid culture）, the recombinant expression and purification of its antigen protein （urease, VacA, CagA and lipopolysaccharide）.