环氧化酶-2选择性抑制剂NS398对紫外线照射损伤人角质形成细胞的保护作用

Photoprotection by the selective COX-2 inhibitor NS398 against UV-mediated damage in keratinocytes

目的探讨环氧化酶-2选择性抑制剂氮-2，环己氧-4，硝基苯-甲基磺胺（NS398）对长波紫外线（UVA）、中波紫外线（UVB）联合照射损伤HaCaT细胞的光保护效应。方法HaCaT细胞传代培养，与含不同浓度NS398的培养基孵育2h后接受紫外线（uV）照射，四甲基偶氮唑（MTT）法测定HaCaT细胞的增殖变化，二氯荧光素醋酸酯（DCFH—DA）荧光探针法测定细胞内活性氧（ROS）。结果细胞存活率测定表明，UV照射的HaCaT细胞，细胞活性明显下降（P〈0．01），NS398能明显提高细胞生存能力并呈浓度依赖性（P〈0．05）。NS398能明显降低UV照射引起的细胞内ROS含量，有效抵抗UV诱导的HaCaT细胞死亡或凋亡，呈浓度依赖性（P〈0．05）。结论NS398通过减少UV辐射诱导的细胞内ROS的生成，提高细胞的生存能力，发挥防护UV辐射损伤的作用。

Objective To investigate photo-protective effect of NS398 on above cells damaged from UV irradiation. Methods HaCaT cell and HaCaT keratinocytes were incubated in the culture medium supplemented with different concentration of NS398 for 2h before diffierent dosages of UV irradiation. MTT assay was used to detect cell proliferation and cellular activity; Fluorospectrophotometer assay was used to measure the change of ROS level in the ceils which were UV-mediated. Results After irradiation with UV, proliferation and cellular activity of HaCaT ceils were decreased （ P 〈 0.05 ） ; NS398 reduced the generation of ROS in ceils induced by UV irradiation apparently and the cell apoptotic rate was dependent on the concentration of NS398. Conclusion NS398 might play an important role in the treatment of UV irradiation injury by decreasing the generation of ROS in ceils after UV irradiation and increasing the survival ability of HaCaT cells.