解聚素-金属蛋白酶17在结肠直肠癌中的表达及其意义

A disintegrin and metalloprotease 17(ADAM17) expression in colorectal cancer and its clinical significance

目的:研究解聚素-金属蛋白酶17(a disintegrin and metalloprotease17,ADAM17)在结肠直肠癌中的表达及其临床意义。方法:收集80例手术切除的结肠直肠癌及60例正常结肠直肠黏膜组织,采用RT-PCR和免疫组化SP法检测ADAM17在结肠直肠癌及正常结肠直肠黏膜组织中的表达情况;分析ADAM17在结肠直肠癌组织中的表达和病人临床病理特征之间的关系。结果:结肠直肠癌组织的ADAM17基因表达高于正常结肠直肠黏膜组织(0.66±0.14比0.26±0.12,P<0.01),ADAM17蛋白在结肠直肠癌组织中之表达阳性率为79%,在正常结肠直肠黏膜组织中之表达阳性率为25%,差异具有统计学意义(P<0.01)。ADAM17高表达与结肠直肠癌浸润深度及远处转移相关(P<0.05);与病人的年龄、性别、肿瘤部位、大小、分化程度及淋巴结转移无关。结论:ADAM17在结肠直肠癌组织中呈高表达,此情况与结肠直肠癌浸润深度及远处转移相关,可能成为一种新的判断结肠直肠癌病人预后的标志物。

Objective To investigate the expression of a disintegrin and metalloprotease 17（ADAM17） in colorectal cancer （CRC）, and its clinical significance. Methods The expression of ADAM17 was detected by RT-PCR and immunohistochemical staining. The relationship between ADAM17 and clinico-pathological parameters of CRC was analyzed. Results ADAM17 gene expression was significantly higher in CRC lesions than that in normal colorectal epithelial tissue（0.66±0.14 vs 0.26_＋0.12,P〈0.01）; the positive rate of ADAM17 protein expression in CRC lesion was 79%, significantly higher than that in normal epithelial tissue（25%）. Significant relationship was found between ADAM17 protein expression and the depth of tumor invasion and metastasis （P〈0.05）. No relationship was found between ADAM17 expression and the age, sex, tumor size, nodal status, grade of differentiation, tumor staging, as well as tumor site. Conclusions ADAM17 mRNA was highly expressed in CRC tissue, its overexpression status was closely related with the depth of tumor invasion and metastasis.ADAM17 might serve as a potential new prognostic marker of CRC.