外周血单个核细胞产生IL-8影响因素的探讨

Evaluation of the Factors Influencing on Interleukin-8(IL-8) Released from Human Peripheral Blood Mononucler Cells

目的：观察Ｃ５ａ、ＰＭＡ、Ａ２３１８７、ＬＰＳ对ＰＢＭＣＩＬ－８产生的影响，为研究减轻Ｃ５ａ致病作用的途径创造条仵。方法：用ｒｈＣ５ａ、ＰＭＡ、Ａ２３１８７、ＬＰＳ诱导ＰＢＭＣ，以ＥＬＩＳＡ法测定细胞培养上清中ＩＬ－８。结果：ＰＢＭＣ经ｒｈＣ５ａ、ＰＭＡ、Ａ２３１８７和ＬＰＳ诱导后ＩＬ－８分泌水平较对照组增高。结论：激活ＰＫＣ、提高细胞内钙离子浓度可使ＩＬ－８分泌增加，提示用ＰＫＣ抑制剂、钙通道拮抗剂有可能使Ｃ５ａ介导的ＰＢＭＣＩＬ－８分泌水平降低。

Objective:Interleukin-8(IL-8) from human peripheral blood mononuclear cells(PBMCs) and stimulated with C5a,PMA,A 23187 and LPS was examinated for finding a way to reduce the pathogensis of C5a.Method:The IL-8 in supernatant of PBMCs which were induced with rhC5a,PMA,A 23187 and LPS was detected by ELISA.Result:The secretion of IL-8 from PBMCs induced with rhC5a,PMA,A 23187 and LPS increased in comparison with the control group.Conclusion:Promotion of PKC or cytoplasma calcium increased IL-8 secretion and PKC inhibitor or calcium channel antagonist decreased IL-8 secretion from PBMCs induced with C5a.