链霉菌702菌株原生质体抗药性致死突变标志紫外诱变筛选研究

The protoplasts resistance-chemicals mutational labelling selection model of the streptomyces 702

实验采用庆大霉素对链霉菌702原生质体致死突变标志的紫外诱变筛选模型,以提高其所产抗真菌生物活性物质的产量。实验结果表明,紫外线处理40 s时,致死率达67.15%,突变率高达20.18%,突变株经过摇瓶筛选获得高产菌株42-23-111,产素单位达到949μg/mL,比出发菌株提高了23.2%。

In order to increase the yield of antifungi substance, UV induced-mutation and the protoplasts resistance-gentamycin mutational labelling selection model was used to screen high-yield mutants of streptomyces 702. The results showed that the gentamycin-resistant mutational frequency of the protoplasts of streptomyces 702 treated by UV with 40 seconds was up to 20.18% and the lethal rate was 67.15%. High antifungi substance-producing mutant 42-23-111 was isolated in the screening experiments, and its yield of antifungi substance reached 949 μg/mL, which was 23.2% higher than that produced by its parent strain.