柿子醇提取物的体外抗氧化研究

In vitro Antioxidant Properties of Extract of Non-astringent Persimmons

目的:研究柿子(甜柿)醇提取物(extracts of non-astringent persimmons,EP)体外抗氧化作用。方法:用70%乙醇制备柿子提取物,并对其主要活性成分的含量进行测定;以BHT、VC、D-甘露醇为阳性对照,测定了EP的总抗氧化活性、还原力、脂质过氧化以及清除DPPH自由基、羟基自由基、超氧阴离子的能力。结果:EP的总糖、多酚及黄酮类含量分别为62.03%、0.33%、0.725%。EP具有较好的总抗氧化活性和总还原性,能抑制脂质过氧化和清除自由基。其对DPPH自由基、羟基自由基、超氧阴离子自由基、脂质过氧化的IC_50分别为0.8、4.1、2.1、4.2mg/ml。结论:EP具有明显的抗氧化活性。

Objective： To study the antioxidant properties of extract of non-astringent persimmons（Diospyos kaki）（EP） in vitro. Method： The extract of non-astringent persimmons were preparated by 70 % alcoholic refluence. The content of the active components of EP was mensurated. The antioxidant activity of the EP has been analyzed by using different assays, such as total antioxidant activity, reducing power, hydroxyl radical scavenging assay, superoxide radical scavenging assay, DPPH radical scavenging assay and lipid peroxidation assay. Antioxidant activities of BHT, VC, and D-mannitol were as a positive control. Result： The contents of total saccharides, total phenolic, and total flavonoid in EP were 62.03 %, 0.33 %, 0.725 %, respectively. EP was proved with total antioxidant activity in evidence, effective reducing power, inhibition of lipid peoxidation and free radical, scavenging activity. The ICs0 values of inhibition for DPPH radical, hydroxyl radical, superoxide anion radical and lipid peroxidation assay were 0.8, 4.1, 2.1 and 4.2 mg/ml, respectively. Conclusion： EP has obvious antioxidant activities in vitro.