摘要
本实验从牛初乳中分离纯化胰岛素样生长因子-Ⅰ(IGF-Ⅰ),重点对分离获取高水平IGF-I的前处理条件进行了较为细致的条件优化,每一步骤中IGF-Ⅰ的质量浓度检测均采用放射免疫法测定。为后续工作中采用超滤法规模化、高水平生产IGF-Ⅰ提供了实验依据。
In this study, the insulin-like growth factor - Ⅰ （IGF- Ⅰ） in bovine colosmuns was separated and purified. Especially, the study focused on the optimization of pretreatment conditions in the separation to access high level of IGF- Ⅰ. The content of IGF- Ⅰ in each process was detected by radio-immunoassay （RIA）. Provide the basis for follow-up work to further purified with scale ultra-filtration and high-level production of IGF-Ⅰ in the future.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第4期154-157,共4页
Food Science
